ρ(P)ρ/(C)动态变化对SBR强化生物除磷系统微生物种群的影响  

Influence of P/C Ratios on the Dynamic Changes of the Organism Community for the Sequencing Batch Reactor (SBR) Enhanced Biological Phosphorus Removal System

作　　者：王亚宜[1] 江帆[1] 张兆祥[1] 李小伟[1] 杨健[1] 高乃云[1] 彭永臻[2] 

机构地区：[1]同济大学污染控制与资源化研究国家重点实验室,上海200092 [2]北京工业大学水质科学与水环境恢复工程重点实验室,北京100124

出　　处：《北京工业大学学报》2011年第5期740-747,共8页Journal of Beijing University of Technology

基　　金：国家自然科学基金资助项目(5060806450628808);全国优秀博士学位论文作者专项基金资助项目(200756);国家教育部新世纪优秀人才支持计划资助项目(NCET-08-0404);上海市科技人才计划资助项目(09QA1406100);国家科技重大专项资助项目(2008ZX07421-0022008ZX07407-007-1)

摘　　要：通过调节进水ρ(P)ρ/(C)的不同水平(2.9/100、1.4/100、0.57/100、0.29/100、1.4/100和2.9/100),考察了A/O-SBR系统强化生物除磷效果的动态变化;同时利用PCR-DGGE分子生物学技术,研究了聚磷菌和聚糖菌种群的竞争与演变.结果表明,当ρ(P)ρ/(C)逐渐降低时(2.9/100→1.4/100→0.57/100→0.29/100),吸收单位碳源的厌氧释磷量逐渐降低,而胞内糖原量逐渐升高.相应的DGGE图谱显示,微生物类群在ρ(P)/ρ(C)降低过程由11 OTUs升高到14 OTUs,最后降至7 OTUs;结合生化指标判断,系统优势菌种呈现的是从聚磷菌占优势、聚糖菌聚磷菌共存到聚糖菌占优势的动态变化.随后,提高进水ρ(P)ρ/(C)值从0.29/100到1.4/100再到2.9/100,污泥吸收单位碳源的厌氧释磷量逐渐升高,而胞内糖原量逐渐降低.这说明当聚糖菌占优势以后,通过调节ρ(P)ρ/(C)可重新培养获得聚磷菌优势系统,但DGGE图谱也显示,此时的聚磷菌优势种群较聚糖菌系统的优势种群已有较大变化,且与先前聚磷菌系统的优势种群也不尽相同.The effect of the different P/C ratios,i.e.,2.9/100,1.4/100,0.57/100,0.29/100,1.4/100 and 2.9/100,on the dynamic changes of the phosphorus removal as well as the organism community was examined in an anaerobic/aerobic sequencing batch reactor（A/O-SBR） system,affiliated with Polymerase Chain Reaction-denaturing Gradient Gel Electrophoresis（PCR-DGGE）.Results showed that when the P/C ratio dropped（2.9/100→1.4/100→0.57/100→0.29/100） gradually,the P-release to substrate-uptake ratios decreased companied by an increase in the intracellular glycogen content.Also,the DGGE profiles showed that with the decrease of the influent P/C ratios the dominant microbial species increased from 11 operation taxonomy units（OTUs） to 14 OTUs,and then dropped to 7 OTUs.Judged from the biochemical characteristics of the SBR,it can be confirmed that the predominant microbial populations in the SBR had changed from PAOs to PAOs and GAOs co-exist,and finally to GAOs.For the last recovery period,the P-release to substrate-uptake ratios increased gradually with the decrease of the intracellular glycogen content,indicating that PAOs can be re-cultivated through regulating the influent P/C ratio,once GAOs had dominated the EBPR system.While,the DGGE profiles showed that the dominant species of PAOs re-cultivated from the GAOs-dominated system was different greatly from both the GAOs-dominated system and the initial PAOs-dominated system.

关 键 词：强化生物除磷 磷碳比 聚磷菌 聚糖菌 PCR-DGGE 序批式反应器 

分 类 号：X703[环境科学与工程—环境工程]