高效液相色谱法测定利夫康洗液中蛇床子素的含量  

Content Determination of Osthole in Lifukang Lotion by HPLC

作　　者：李鹏[1] 陈琴华[1] 朱军[1] 彭林[1] 

机构地区：[1]湖北医药学院附属东风医院药剂部,湖北十堰442008

出　　处：《医药导报》2011年第6期785-786,共2页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(基金编号:2009ZRY793)

摘　　要：目的 建立测定利夫康洗液中蛇床子素含量的高效液相色谱（HPLC）法.方法 以日本DL-C18柱（4.6 mm×150 mm,5 μm）为色谱柱,甲醇-水 （65：35）为流动相,检测波长322 nm,流速为1.0 mL·min-1.结果 蛇床子素在浓度为2～100 μg·mL-1范围内有良好的线性关系,相关系数为0.999 5,精密度相对标准偏差（RSD）为1.52%,平均加样回收率为99.7%,RSD为1.12%.结论 该方法操作简单、快速、准确,可用于利夫康洗液中蛇床子素的含量测定.Objective To establish a method for detecting osthole in lifukang lotion by RP-HPLC. Methods RP- HPLC was performed on DL-C18 column （4.6 mm×150 mm,5 μm） with methanol：water （65 ： 35） as mobile phase at flowing rate of 1.0 mL·min-1. The detection wavelength was 322 nm. Results The liner range of osthole was over 2-100μg·mL-1 , r=0. 999 5. The precision expressed as the relative standard deviation （RSD） for the method was 1.52%. The mean extraction recovery was 99.7% ,RSD was 1. 12%. Conclusion The method is proved to be accurate, sensitive and reliable, which is suitable for the determination of osthole in lifukang lotion.

关 键 词：利夫康洗液 蛇床子素 色谱法 高效液相 含量测定 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]