舒芬太尼镇痛对家兔β-内啡肽和氧自由基的影响  

Effects of sufentanil on β-endorphin and oxygen free radical in rabbit

作　　者：杜化娟[1] 王建荔[1] 张明慧[1] 

机构地区：[1]广西医科大学附属肿瘤医院麻醉科,南宁市530021

出　　处：《临床麻醉学杂志》2011年第5期501-503,共3页Journal of Clinical Anesthesiology

摘　　要：目的观察静脉或硬膜外注射舒芬太尼对福尔马林致痛家兔外周血 β-内啡肽（13-EP）和氧自由基（OFR）表达的影响。方法选择18只健康新西兰大白兔随机均分为三组（n=6）：静脉镇痛组（A组）、硬膜外镇痛组（B组）、生理盐水对照组（C组）。每组家兔均予足底注射5％福尔马林0．5ml致痛，致痛后A组和B组分别经静脉和硬膜外注射舒芬太尼0．46 βg／kg和O．64 βg／kg，C组静脉注射等容量生理盐水。记录注药后5、10、15、20、25、30、35、40、45min家免的疼痛评分；检测致痛前（To）、注药后10、20、40min、2、8、24和48h（T1～T7）血清中 β-EP含量；根据血清中丙二醛（MDA）含量评估OFR水平。结果与To时比较， β-EP含量在A、B组R～rr7时、C组T1～T7时均升高（P〈O．05），MDA含量在T1～T7时A、B组降低，C组升高（P〈o．05）；与C组注药后相应时点比较，A．B组疼痛评分，血清伊EP和MDA含量均低于c组（P〈o。05）。结论静脉或硬膜外注射舒芬太尼可有效抑制福尔马林致痛家兔 β-EP含量和OFR表达。Objective To observe the influence of intravenous or epidural analgesia with sufentanil on β-endorphin （ β-EP） and oxygen free radical （OFR） expression in rabbits with formalin-induced pain. Methods Eighteen healthy rabbits were equally randomized into three groups （n= 6）： intravenous analgesia group （group A）, epidural analgesia group （group B） and control group （group C）. All rabbits were injected with 5% formal 0. 5 ml to establish formalin-induced pain model. Then sufentanil 0. 46 μg/kg and 0. 64/,g/kg were intravenously and epidurally injected in group A and group B, respectively. The same volume of saline was intravenously injected in group C as control. Pain scores were recorded every 5 rain from 5 rain after administration to 45 rain after administration The level of ffEP was detected before formalin injection （To）and at 10 min, 20 min, 40 min, 2, 8, 24 h and 48 h （T1-T7） after sufentanil or saline injection, and the level of OFR was evaluated through the level of MDA. Results Compared with To, the level of β-EP increased at T2-T7 in group A and group B and at T1-T7 in group C （P〈0. 05）. The level of MDA at T1-T7 decreased in group A and group B and increased in group C. Compared with group C at the same time points, pain scores,and the levels of β-EP and MDA were lower in group A and group B than in group C （P〈0. 05）. Conclusion Intravenous or epidural analgesia with sufentanil may effectively inhibit the expression of β-EP and OFR in rabbits with ormalinindueed pain.

关 键 词：舒芬太尼 镇痛 疼痛评分 Β-内啡肽 氧自由基 

分 类 号：R965[医药卫生—药理学]