大鼠肝移植不同供肝切取技术对供肝质量的影响  

Influences of different techniques for liver graft procurement on the quality of donor liver in rats with liver transplantation

作　　者：赵宏峰[1] 王宇[1] 任旋磊[1] 周杰[1] 

机构地区：[1]南方医科大学附属南方医院肝胆外科,广州市510515

出　　处：《实用医学杂志》2011年第10期1745-1747,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:2007B031514004)

摘　　要：目的:探讨在大鼠肝移植供肝切取过程中不同方法对供肝质量的影响。方法:SD大鼠120只,随机分成3组,即标准法组(A组)、快速法组(B组)和改良快速法组(C组),每组各行20对大鼠原位肝移植,观察术中资料和术后3周存活率及冷灌洗后肝脏形态学改变。结果:在供肝切取手术时间上C组明显较A、B两组为短(P<0.05),其术后3周存活率也高于A组(P<0.05)。光镜下C组与A、B两组相比肝窦内未见红细胞。结论:标准法和快速法适合于刚建立大鼠肝移植模型的单位,为提高供肝质量、改善术后存活率应选择改良快速法。Objective To study the influence of different techniques for liver graft procurement on the quality of donor liver in rat with liver transplantation.Methods 120 SD rats were divided into 3 groups,the control group with standard liver procurement（group A）,the treatment group with rapid liver procurement（group B）,and the treatment with modified rapid liver procurement（group C）.Liver transplantations were performed for 20 pairs of rats in each group.The morphological change of liver after lavaging and the survival rate were observed during the operation and three weeks after operation.Results The operation time of procuring donor liver in group C was shorter than that in group A and B（P 0.05）and the survival rate after operation for three-week in group C was higher than that in group A（P 0.05）.There was no erythrocyte in hepatic sinusoid of group C under the optical microscope compared to group A and group B.Conclusion The standard and rapid liver procurement method are suitable for the units in which model were established for a while.The modified rapid liver procurement method should be chosen to improve the quality of donor liver and the survival rates after operation.

关 键 词：肝移植 大鼠 供体 肝切取技术 

分 类 号：R657.3[医药卫生—外科学]