强精煎对小鼠睾丸生精细胞凋亡相关基因表达影响的研究  

Experimental Study of Effects of "Qiangjing Decoction" on Expression of Genes Related Apoptosis of Spermatogenic Cells in Model-mouse's Testis

作　　者：宾彬[1] 王杰[1] 陈定雄[1] 莫秋柏[1] 徐杰新[1] 崔锦珠[1] 

机构地区：[1]广西中医学院第一附属医院,广西南宁530023

出　　处：《辽宁中医药大学学报》2011年第6期10-12,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：广西中医学院自然科学基金项目(Y2007023);广西壮族自治区卫生厅计划课题(Z2007111);广西壮族自治区教育厅面上项目(200610MS191)

摘　　要：目的:观察强精煎对实验小鼠睾丸Fas、Fasl、Bcl-2和Bax蛋白表达的影响。方法:将80只健康雄性昆明种小鼠分为正常组、模型组、强精煎组、黄精赞育胶囊组,以环磷酰胺注射液40mg/kg腹腔注射制造小鼠生精功能障碍模型,以HE染色观察小鼠睾丸病理形态,采用Johnson评分评价精子发生和发生障碍程度,用免疫组织化学SABC法检测Fas、Fasl、Bcl-2和Bax蛋白在小鼠睾丸实质组织中的表达率。结果:模型组小鼠睾丸组织可见明显的病理损伤,强精煎组则生精功能良好,黄精赞育胶囊组稍差。与正常组比较,模型组Johnson评分偏低,且Fas和Fasl蛋白表达率偏高(P<0.01),强精煎组和黄精赞育胶囊组与正常组各方面差异均无统计学意义(P>0.05)。与模型组比较,正常组、强精煎组和黄精赞育胶囊组睾丸Johnson评分偏高,Fas、Fasl和Bax蛋白表达率显著降低(P<0.01),Bcl-2表达率显著升高(P<0.01),细胞内Bcl-2/Bax比值显著升高(P<0.01)。结论:强精煎可以抑制Bax、Fas和Fasl,并促进Bcl-2蛋白在受损伤小鼠睾丸实质组织中的表达。Objective：To study the effects of＂Qiangjing Decoction＂on the expression of Fas,Fasl,Bcl-2 and Bax related apoptosis of spermatogenic cells in model-mouse’s testis.Methods：The object of 80 healthy male rats was assigned into four randomized blocks,one as treat group fed by Qiangjing Decoction,the other as control groups included normal control group with no disposal,experimental control group namely model group,and standard control group fed by Huangjing Zanyu Jiaonang.Maked the model of spermatogenic disorder by cyclophosphamide,had the mice’s testis dyed with the method of Haematovilin-Eosin（H-E）dyeing for histopathological examination,evaluated the degree of normal and deranged spermatogenesis according to Johnson score method and tested the expression of Fas,FasL,Bcl-2 and Bax System by Streptavidin-Biotin Complex（SABC）method of immunohistochemistry.Results：Compared with the normal group,the Johnson score of the model group was prominently decreased and the percentages of Fas ＆ Fasl was prominently increased（P〈0.01）,however,the treat group and the standard group have no significant differences in all areas.Compared with the model group,the normal group,the treat group and the standard group were prominently increased on the Johnson score,the percentages of Bcl-2 and the ratio of Bcl-2/Bax,and were prominently decreased on Fas,Fasl and Bax（P〈0.01）.Conclusion：Qiangjing Decoction was able to inhibit the expression of Fas,FasL and Bax in the testis of model-mice with spermatogenic disorder,and promote the expression of Bcl-2.

关 键 词：强精煎 生精功能障碍 实验研究 细胞凋亡 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]