秦巴山区野板栗居群遗传多样性AFLP分析  

Genetic Diversity on Wild Populations of Chestnut in Qinba Mountain Area of West China with AFLP Markers

作　　者：艾呈祥[1] 沈广宁[1] 张凯[2] 田寿乐[1] 许林[1] 

机构地区：[1]山东省果树研究所,泰安271000 [2]山东英才学院农学院,济南250104

出　　处：《植物遗传资源学报》2011年第3期408-412,共5页Journal of Plant Genetic Resources

基　　金：山东省农业良种工程(2010lz010);国家科技支撑计划(2008BAD92B03)

摘　　要：利用荧光AFLP标记技术对来自秦巴山区的野板栗10个居群共262个单株进行遗传多样性研究。10对AFLP引物共扩增出1297条谱带,其中多态性位点数1011个,多态位点百分率为77.95%;Ne′is基因多样性指数为0.1439~0.2046,总体为0.2518;Shannon信息指数的变异范围为0.1972~0.2895,总体为0.4089;甘肃地区野板栗居群遗传多样性水平最高,陕西宝鸡居群的遗传多样性水平最低。AMOVA分析表明野板栗居群间的遗传变异占总变异的17.51%,居群内变异占69.76%。UPGMA聚类可将供试10个居群划分为3类,聚类结果表现出明显的地域性。Genetic diversity of 262 single individual plants representing 10 wild populations,which were derived from Qinba mountain area of West China,was assessed with Fluorescent AFLP Technique.A total 1297 fragments were generated from 10 pairs of selective AFLP primer combinations,1011 of them were polymorphic.The level of polymorphism was 77.95%.Nei′s gene diversity index ranged from 0.1439 to 0.2046 for different populations,with the total value of 0.2518.Shannon information index ranged from 0.1972 to 0.2895,with the total value of 0.4089.The chestnut populations from Gansu district had the highest genetic diversity,however,those from Baoji Shaanxi district were the lowest.The result of AMOVA analysis showed that the genetic variation within populations was 69.76% and the variation ameng populations was 17.51%.Ten populations were clustered into 3 groups by UPGMA dendrogram,which was significantly correlated with the geographic distribution of the materials.

关 键 词：板栗 野生居群 遗传多样性 AFLP 

分 类 号：S664.2[农业科学—果树学]