SSR与SRAP标记在玉米品种鉴定中的比较研究  

Comparison of SSR and SRAP Marker for Varieties Identification in Maize(Zea mays L.)

作　　者：盖树鹏[1] 盖伟玲[2] 黄进勇[1] 

机构地区：[1]青岛农业大学生命科学学院,青岛266109 [2]青岛农业大学农学与植保学院,青岛266109

出　　处：《植物遗传资源学报》2011年第3期468-472,共5页Journal of Plant Genetic Resources

基　　金：山东省中青年科学家奖励基金项目(2004bsb014)

摘　　要：利用SSR标记和SRAP标记对19个玉米品种及8份莱农15样品进行了分析,比较了2种标记的分辨能力及在亲缘关系和杂交种纯度鉴定中的表现。与SSR标记相比,SRAP标记用于玉米品种鉴定扩增位点数量更多,PIC值更高,具有更高的分辨率;在亲缘关系分析方面,SSR检测的遗传距离变幅更大,2种标记计算的遗传距离呈极显著正相关,分类结果基本一致;在杂交种纯度检测中,SRAP标记的期望位点在杂交种群体中检测率高于SSR标记相应位点,检测杂交种纯度结果更接近田间种植鉴定,因而准确度更高。SRAP在玉米品种鉴定中具有一定的优势,可作为SSR标记技术的有益补充,特别是在杂交种纯度鉴定中应用。SSR marker is usually the prior candidate of molecular marker for seed identification in maize.Unfortunately,some problems still exist in hybrids purity identification.Consequently,supplementary markers are still necessary to be explored to facilitate varieties identification besides SSR marker.In this study,SSR and SRAP markers were performed in 19 maize varieties and 8 samples of Lainong 15.The discriminative power of the markers,genetic relationship and hybrids purity revealed by SSR and SRAP markers were compared and analyzed.Comparing to SSR markers,SRAP markers displayed more amplified alleles per primer-pair,higher PIC value,and higher discriminative power.For the 19 maize varieties,genetic distance estimated based on SSR and SRAP data were significantly correlated with each other,and similar clustering results were achieved.Specific primers of SSR and SRAP with complementary pattern were used to detect hybrid purity of Lainong 15.The hybrid purity identified by SRAP marker was more vicinal to field test than that by SSR marker.In conclusion,SRAP markers could be applied in maize identification as a useful supplement of SSR,especially in hybrid purity identification.

关 键 词：玉米 SSR SRAP 品种鉴定 

分 类 号：S513[农业科学—作物学]