西北油松叶片δ^(13)C特征与环境因子和叶片矿质元素的关系  

Relationships between foliar stable carbon isotope composition and environmental factors and leaf element contents of Pinus tabulaeformis in northwestern China

作　　者：李善家[1] 张有福[2,3] 陈拓[3] 

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050 [2]河南科技大学农学院,洛阳471003 [3]中国科学院寒区旱区环境与工程研究所冰冻圈科学国家重点实验室,兰州730000

出　　处：《植物生态学报》2011年第6期596-604,共9页Chinese Journal of Plant Ecology

基　　金：国家自然科学基金项目(30770342);公益性行业科研专项(200806036)资助

摘　　要：选取甘肃、宁夏及内蒙古油松(Pinus tabulaeformis)10个天然种群,通过测定油松叶片稳定碳同位素组成(δ13C)和元素含量等特征,分析了油松叶片δ13C特征及其与环境气候因子和叶片矿质元素含量之间的关系。研究结果表明,油松叶片δ13C的变化范围在–28.68‰––25.02‰之间,平均值为–26.82‰;油松叶片δ13C值与海拔、经度之间相关性不显著,与纬度之间呈显著正相关,与年降水量、年平均温度之间均呈显著负相关,说明年平均温度和年降水量是决定油松13C分馏能力差别及生长的主要限制因子;叶片元素N、P、K、Si、Ca、Fe含量与δ13C值有明显的相关性,其中叶片N、P、K含量与δ13C值显著负相关,Si、Ca、Fe含量与δ13C值显著正相关,可见不同种群间δ13C组成差异可反映植物营养元素含量的变化状况。不同生境条件下环境气候因子和矿质元素含量的这种响应模式在一定程度上反映和影响了以油松为建群种和优势种的生态系统的δ13C变化特性。Aims Our objective was to analyze relationships between foliar stable carbon isotope composition （δ13C） and environmental factors and leaf element contents in Pinus tabulaeformis, which is widely distributed in northwestern China. Methods Leaf samples were taken in ten natural populations of P. tabulaeformis from Gansu, Ningxia and Inner Mongolia. The δ13C was determined using an isotope mass spectrometer. Environmental factors were recorded by Magellan GPS320 and from nearby weather stations. Leaf element contents were measured with low vacuum scanning electron microscope and X-ray energy dispersive spectroscopy. Carbon content was estimated with the potassium dichromate method, and nitrogen content was measured with the micro-Kjeldahl method. Data were evaluated with Excel 2003 and SPSS 13.0 analysis of correlation and regression. Important findings Foliar δ13C ranged from –28.68‰ to –25.02‰, with an average value of –26.82‰. Foliar δ13C did not closely correlate with altitude and longitude, but was significantly positively correlated to latitude and negatively correlated with annual mean precipitation and temperature. Therefore, δ13C in different natural populations of P. tabulaeformis is significantly influenced by environmental factors, and annual mean temperature and precipitation are essential factors in δ13C’s capability of fractionation and growth. Leaf N, P and K content were significantly negatively correlated with δ13C; however, Si, Ca and Fe content were significantly positively correlated with δ13C. Different δ13C in different natural populations reflects changes in plant nutrient element content. Response mode of climatic factors and element content under different habitat conditions reflected and affected properties of δ13C change in natural populations of P. tabulaeformis.

关 键 词：相关性 环境因子 叶片元素含量 油松 稳定碳同位素组成 

分 类 号：S791.254[农业科学—林木遗传育种]