曼氏血吸虫表膜两型转糖蛋白在日本血吸虫表膜的免疫细胞化学定位  

IMMUNOCYTOCHEMICAL LOCALIZATION OF TWO FACILITATED GLUCOSE TRANSPORTERS OF SCHISTOSOMA MANSONI IN THE TEGUMENT OF SCHISTOSOMA JAPONICUM

作　　者：江家婉[1] 钟慈声[1] 齐玲 俞永富 

机构地区：[1]上海医科大学基础医学院生物物理教研室,上海200032

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第4期222-224,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：卫生部青年回国启动基金

摘　　要：目的： 观察曼氏血吸虫表膜两型转糖蛋白（ Ｓ Ｇ Ｔ Ｐ１ 和 Ｓ Ｇ Ｔ Ｐ４） 分子在日本血吸虫成虫表膜上的分布，确定日本血吸虫和曼氏血吸虫的 Ｓ Ｇ Ｔ Ｐ 是否有同源性。方法： 采用超薄切片技术制备日本血吸虫超薄冷冻切片。在电镜下观察曼氏血吸虫的 Ｓ Ｇ Ｔ Ｐ１ 和 Ｓ Ｇ Ｔ Ｐ４ 抗体对日本血吸虫的相应抗原的免疫标记定位。结果： 免疫定位显示 Ｓ Ｇ Ｔ Ｐ１ 定位于日本血吸虫成虫表膜基底部质膜及其反褶的质膜上， Ｓ Ｇ Ｔ Ｐ４ 定位于成虫表膜顶部质膜及其内陷质膜上。结论： 两型转糖蛋白分子在曼氏血吸虫和日本血吸虫表膜上的定位相同。表明此两种血吸虫两型表膜的转糖蛋白分子有很大同源性。AIM: To observe the distribution of Schistosoma mansoni glucose transport proteins, SGTP1 and SGTP4, in the tegument of Schistosoma japonicum . METHODS: The rapidly frozen fixation technique and ultracryomicrotomy were adopted for preparing ultrathin cryosections of S\^japonicum . Anti\|SGTP1 and anti\|SGTP4 antibodies were used to localize the corresponding antigens in the tegument of adult S\^japonicum by immunocytochemical technique. RESULTS: SGTP1 was localized on the basal membrane of the tegument and its infoldings, SGTP4 was localized on the apical membrane of the tegument and its invaginations of S\^japonicum . CONCLUSION: The same localization for SGTP 1 and SGTP4 in the tegument of S\^japonicum and S\^ mansoni exhibited apparent homology between SGTPs of the two schistosomes.

关 键 词：日本血吸虫 转糖蛋白 免疫细胞化学 

分 类 号：R383.24[医药卫生—医学寄生虫学]