人芽囊原虫的形态观察及致病性研究  

RESEARCH ON MORPHOLOGY AND PATHOGENICITY OF BLASTOCYSTIS HOMINIS

作　　者：贺丽君[1] 苏云普[1] 颜秋叶[1] 朱新朋[1] 刘辉[1] 

机构地区：[1]河南省卫生防疫站,郑州450003

出　　处：《中国寄生虫病防治杂志》1999年第3期195-196,共2页Chinese Journal of Parasitic Disease Control

摘　　要：为探讨人芽囊原虫的形态及其致病性，采用３０°斜面鸡蛋培养基对人芽囊原虫进行体外培养，在光学显微镜和扫描电镜下观察其形态和致病性。在形态观察中可见空泡型、阿米巴型和颗粒型 ３ 种形态，并证实有出芽生殖和颗粒形虫体增殖现象。以不同数量培养４８ ｈ 的圆形空泡型虫体感染小白鼠观察其致病性。结果显示， Ｄ 组（空白对照组）和 Ａ 组（感染数为 １０４ ／只）在肠粘膜上未发现虫体； Ｂ组（感染数为２０４ ／只）和 Ｃ组（感染数为 ３０４ ／只）在肠粘膜上发现虫体并能够致肠粘膜损伤，提示对肠粘膜损伤的轻重程度与人芽囊原虫感染数量有关。In order to research the morphology and pathogenicity of Blastocystis hominis , it was cultivated by egg culture medium with an 30° angle in vitro and was observed under light and scan EM for its morphological structure and pathogenicity. Three forms of morphological structure were found, vacuolated form, amoebic form and granular form. Furthermore, confirmations of budding reproduction and proliferation of granular parasite body were examined. Using varies amount of spherical vacuolated form parasites cultivated 48 h to infect mice, the result showed that the parasite bodies were not found in intestinal mucosa at the control group and the group infected with 10 4 parasites each mouse; however, parasites were found in the groups infected with 20 4 and 30 4 parasites each nouse and caused injuries of intestinal mucosa. It was suggested that the impairment of intestinal mucosa was in line with the amount of infected Blastocystis hominis .

关 键 词：人芽囊原虫 形态 致病性 

分 类 号：R382.9[医药卫生—医学寄生虫学]