α-双炔失碳酯诱导阿霉素抗药的MCF-7乳癌细胞凋亡  

Induction of apoptosis by α anordrin in adriamycin resistant MCF 7 breast cancer cells

作　　者：楼丽君[1] 胥彬[2] 

机构地区：[1]浙江省衢州市人民医院,衢州324000 [2]中国科学院上海药物研究所,上海200031

出　　处：《河南医科大学学报》1999年第3期37-39,共3页Journal of Henan Medical University

摘　　要：目的 :研究α 双炔失碳酯 (α anordrin ,ANO)对阿霉素抗药的MCF 7乳癌细胞 (MCF 7adr细胞 )的凋亡诱导作用。方法 :采用形态学、DNA琼脂糖凝胶电泳、流式细胞术等方法。结果 :ANO 10～ 5 0 μmol/L作用 4 8h明显抑制MCF 7adr细胞的生长 ,诱导MCF 7adr细胞核碎裂 ,染色质凝缩 ,电泳显示核小体之间DNA断裂。流式细胞术检测发现经ANO作用后约有 2 6%的细胞出现在“亚G1期”。结论 :ANO能够诱导MCF 7adr乳癌细胞凋亡 。Aim:To investigate the apoptosis inducing effect of α anordrin(ANO) on adriamycin resistant MCF 7 breast cancer cells (MCF 7 adr ).Methods: Morphology assessment,DNA agarose gel electrophoresis,and flow cytometry were used in the study.Results: It was found that ANO 10～50 μmol/L significantly inhibited the growth of MCF 7 adr cells, induced the break of nuclei and the condensation of chromatin,and led to the fragmentation of internucleosomal linker DNA.About 26% of total cells treated with ANO was found to accumulate at the “sub G 1 peak” as determined by flow cytometry. Conclusion: ANO can effectively induce the apoptosis of MCF 7 adr cells and has no cross resistance to adriamycin.

关 键 词：阿霉素 Α-双炔失碳酯 多药抗药性 乳癌细胞凋亡 

分 类 号：R979.21[医药卫生—药品] R979.14[医药卫生—药学]