一株极端耐盐曲霉的分离、鉴定及生物学特性分析  

Isolation,Identification and Biologic Characteristics of an Extreme Halotolerant Aspergillus sp.

作　　者：刘晓丹[1,2] 刘金亮[1] 魏毅[1] 田玉平[1] 范飞飞[1] 潘洪玉[1] 张世宏[1] 

机构地区：[1]吉林大学植物科学学院,长春130062 [2]吉林农业科技学院生物工程学院,吉林吉林132101

出　　处：《吉林大学学报（理学版）》2011年第3期548-553,共6页Journal of Jilin University:Science Edition

基　　金：国家自然科学基金(批准号:30871607)

摘　　要：从晒盐场盐池周边的风干野生植被表面分离筛选到1株极端耐盐菌株CCHA,通过形态观察及ITS序列分析对菌株进行鉴定,并对其生物学特性、耐盐性及金属耐受性进行分析.研究结果表明:菌株CCHA与21株己报道的曲霉属菌株同源性为55.9%～100%;其生长温度范围为25～45℃,最适温度为35℃;生长pH值范围为2.5～12.0,最适pH值为5.0～8.0;生长盐度范围为0～31%,最适盐度为5%;对Cu2+有较强的耐受性.鉴定该菌株为曲霉菌(Aspergillus sp.).A halo-tolerant strain CCHA was isolated from the surface of wild vegetation around saltern,identified by the morphological observation and ITS sequence analysis,and their biological characteristics,salt-resistance and metal-tolerance were analyzed.The homology of ITS sequence between CCHA and each of other 21 Aspergillus strains which have been reported was 55.9%—100%;growth temperature range of it was from 25 ℃ to 45 ℃,the optimum at 35 ℃;growth pH range of it was from 2.5 to 12.0,the optimum at 5.0—8.0;salinity range of it was 0—31%,the optimum at 5%;and the strain exhibited higher tolerance to Cu2＋.CCHA strain was identified as Aspergillus sp.

关 键 词：耐盐曲霉 分离 鉴定 转录间隔区(ITS) 盐度 PH值 金属耐受性 

分 类 号：Q939[生物学—微生物学]