野生兰科植物菌根内生真菌分离及鉴定  

Isolation and Identification of Endophytic Fungi in Wild Orchid Mycorrhiza

作　　者：吴剑丙[1] 朱江敏[1] 白坚[1] 李玉婷[1] 张克铭[1] 王慧中[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2011年第3期228-232,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(30870180;31070298);浙江省重大科技专门项目(2008C12081);杭州市科技计划项目(20080432T06;20101032B25)

摘　　要：利用石蜡切片技术对实验室收集保存的25种野生兰科植物的菌根结构进行显微观察,并采用常规分离方法,对内生真菌进行分离及菌落形态和分子生物学鉴定.结果表明:实验室保存的兰科植物具有典型的菌根形态;从菌根中共分离获得82株真菌菌株,分属于镰孢属(Fusarium Link)、毛壳属(Chaetomium Kunze exFr.)和木霉属(Trichoderma Persoon)等11属,其中镰孢属真菌占70.7%.Using the way of paraffin section, the experiment observed the microstructures of mycorrhizas of 25 kinds of wild orchids, which were collected and kept in the lab, and isolated the endophytic fungi, indentified colonial morphology by conventional isolation method. The results show that the orchids kept in the lab are the typical mycorrhizal plants. 82 isolated fungi strains belong to 11 genera, such as Fusarium, Chaetomium, Trichoderrna, etc, and about 70. 7% of them belong to Fusarium.

关 键 词：兰科植物 菌根 内生真菌 分离鉴定 

分 类 号：Q939.5[生物学—微生物学]