金属卟啉标记物在化学发光免疫检测中优势的理论分析  

Theoretical Analysis on the Ascendancy of Metalloporphyrins as the Label in the Chemiluminescence Immunoassay

作　　者：陈灿玉 袁通[1] 张现侠[1] 黄志坚[1] 

机构地区：[1]杭州师范大学生物医药与健康研究中心,浙江杭州311121

出　　处：《杭州师范大学学报（自然科学版）》2011年第3期233-236,共4页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省重大科技专门项目(2008C12063);杭州市科技发展计划项目(20100933B10);杭州师范大学科研项目(YS05203133)

摘　　要：为阐述金属卟啉配合物替代辣根过氧化酶作为标记物进行化学发光免疫检测的优势,文章构建了简单的化学发光免疫检测模型,分析了化学发光免疫反应中的平衡方程.结果表明:金属卟啉配合物作为标记物在理论上可以提高信噪比2个数量级以上;与酶标法相比,高纯度的金属卟啉标记抗体大大降低了非特异吸附本底信号和检测体系的系统误差,极大提高了化学发光免疫检测的灵敏度和准确度.In order to illustrate the ascendancy of metalloporphyrins as the label in the chemiluminescence immunoassay instead of HRP, the paper constructed simple model of chemiluminescence immunoassay, and analyzed the balance equations in the reaction. The results show that the signal-noise ratio can increase over two orders of magnitude with the metalloporphyrins as the label in theory. Comparing with the enzyme linked immunosorbent assay, highly purified metalloporphyrins can much decrease the non-specific adsorbed background signals as well as the systematic error of the detection, and greatly improve the sensitivity as well as the accuracy of the chemiluminescence immunoassay.

关 键 词：金属卟啉 标记 化学发光 免疫分析 

分 类 号：R392-33[医药卫生—免疫学]