盐酸万乃洛韦体外抗单纯疱疹病毒活性的测定  

Determination of Antivirus Activity of Valaciclovir Hydrochloride to Herpes Simplex Virus in Vitro.

作　　者：赖伟红[1] 韩国柱[1] 钟铭英[1] 叶顺章[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042

出　　处：《中国皮肤性病学杂志》1999年第5期269-270,共2页The Chinese Journal of Dermatovenereology

摘　　要：目的　评价盐酸万乃洛韦的细胞毒性和抗单纯疱疹病毒活性。方法　用细胞培养法测定盐酸万乃洛韦和阿昔洛韦对 Ｖｅｒｏ 细胞的细胞毒性和对６ 株单纯疱疹病毒（ 其中Ⅰ型和Ⅱ型各３ 株） 的抗病毒活性。结果　盐酸万乃洛韦对 Ｖｅｒｏ 细胞的半数中毒浓度（ Ｔ Ｄ５０） 为２９２ ．７１μｍ ｏｌ／ Ｌ；对 Ｈ Ｓ Ｖ１ 和 Ｈ Ｓ Ｖ２ 的平均半数有效浓度（ Ｉ Ｃ５０） 分别为０ ．１１１μｍ ｏｌ／ Ｌ 和０ ．１６９μｍ ｏｌ／ Ｌ；对 Ｈ Ｓ Ｖ１ 和 Ｈ Ｓ Ｖ２ 的抗病毒治疗指数分别为２６４０ 和１７３１ 。结论　万乃洛韦是一种细胞毒性小的有效的抗 Ｈ Ｓ Ｖ 药物。Objective To evaluate the cytotoxicity and antiherpesvirus activity of valaciclovir hydrochloride.Methods The cytotoxicity to Vero cell and antivirus activity to 3 strains of herpes simplex virus type1(HSV 1) and 3 strains of herpes simplex virus type 2(HSV 2) of valaciclovir hydrochloride were detected in vitro.Results The 50% toxicity dose(TD 50 ) to Vero cell was 292.71μmol/L;the average 50% inhibitory concentrations (IC 50 s) to HSV 1 and HSV 2 was 0.111μmol/L and 0.169μmol/L,respectively,the therapeutic indexes for HSV 1 and HSV 2 were 2 640 and 1 731,respectively.Conclusion It is indicated that valaciclovir hydrochloride in an effective anti HSV compound with low cytotoxicity.

关 键 词：体外实验 万乃洛韦 单纯疱疹病毒 抗病毒活性 

分 类 号：R986[医药卫生—药品] R978.7[医药卫生—药学]