浓核病毒(镇江株)感受性家蚕品种中肠组织不同部位蛋白质比较分析  

Comparative analysis of proteins extracted from different parts of midgut of silkworm susceptible to Bombyx mori densovirus(Zhenjiang Strain)

作　　者：郭锡杰[1,2] 蒋云峰[1] 裘智勇[2] 刘挺[2] 覃光星[2] 沈兴家[2] 

机构地区：[1]江苏科技大学生物与化学工程学院,江苏镇江212018 [2]中国农科院蚕业研究所农业部蚕桑遗传改良重点开放实验室,江苏镇江212018

出　　处：《江苏科技大学学报（自然科学版）》2011年第2期178-182,199,共6页Journal of Jiangsu University of Science and Technology:Natural Science Edition

基　　金：国家重点基础研究发展计划"973"基金资助项目(2005CB121004);江苏省自然科学基金企业博士创新基金资助项目(SBK200930217)

摘　　要：为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后3个区段的组织蛋白进行了比较分析.结果表明:中肠前、中、后3个区段组织蛋白的点数、位置、点形等差异较小.在中肠前部和后部组织中找出的4个差异蛋白点,分别为脂蛋白前体、囊膜氢离子泵ATP合酶B亚基、α胰蛋白酶A链和肌动蛋白;在中肠中部找出的一个差异蛋白点为Kun itz型胰蛋白酶抑制剂A链.这些蛋白点可能在BmDNV-ZJ侵染感受性家蚕中肠组织细胞中起一定的作用.To understand the proteomic mechanism of susceptibility of silkworm to Bombyx mori densovirus（Zhenjiang Strain,BmDNV-ZJ） and the pathogenesis of silkworm densonucleosis,the proteins extracted separately from the anterior,middle and posterior parts of midgut are comparatively analyzed with the isoelectric focusing electrophoresis（IFE）,SDS-PAGE,2D Platinum software and MALDI-TOF MS.IFE and SDS-PAGE reveal less difference in the number,location and shape of the protein spots on the 2D images among the anterior,middle and posterior part of midgut.Only four differentiated spots are distinguished in the anterior and posterior parts of midgut.They are,respectively,matched to be lipoprotein precursor,vacuolar ATP synthase subunit B,chain A of alpha trypsin and actin.Another differentiated spot is distinguished in the middle part of midgut,being matched with chain A complex of kunitz trypsin inhibitor.These differentiated proteins are speculated to be related to infection of silkworm midgut with BmDNV-ZJ.

关 键 词：家蚕 浓核病毒(镇江株) 中肠 蛋白质 

分 类 号：Q965.8[生物学—昆虫学]