北部湾3种金线鱼属鱼类COI基因序列的比较分析  

Sequence analysis of mitochondrial COI gene in three Nemipterus species from Beibu Gulf

作　　者：董丽娜[1,2] 黄梓荣[1] 艾红[1] 卢伟华 李希国 李娜娜[1,4] 李夏[1] 李永振[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]东莞市海洋与渔业环境监测站,广东东莞523079 [4]大连海洋大学海洋工程学院,辽宁大连116023

出　　处：《中国水产科学》2011年第3期508-514,共7页Journal of Fishery Sciences of China

基　　金：国家科技基础性工作重点项目(2003DEA6N042);科技部社会公益研究专项资金项目(2004DIB3J098);广东省科技计划项目(2005B31001005;2010B03080008);国家财政专项项目(1999-2001)

摘　　要：运用直接测序法对产自北部湾的日本金线鱼(Nemipterus japonicus)、红棘金线鱼(N.nemurus)和苏门答腊金线鱼(N.mesoprion)3种金线鱼属鱼类的线粒体DNA细胞色素氧化酶I(COI)基因序列进行了测序分析,并探讨了3种金线鱼之间的亲缘关系。在所分析的48条COI同源序列片段(696 bp)中共检测到29个单倍型和104个核苷酸多态位点。单倍型分析结果显示,日本金线鱼和红棘金线鱼之间有明显的基因交流。序列差异分析和遗传距离比较结果显示,日本金线鱼与红棘金线鱼之间亲缘关系较近,无明显遗传分化。利用最大简约法(MP)和邻接法(NJ)构建的系统发育树显示,日本金线鱼和红棘金线鱼混杂聚为一支,而苏门答腊金线鱼单独聚为一支。研究结果表明,日本金线鱼与红棘金线鱼的亲缘关系较近,二者可能为同种,而苏门答腊金线鱼则与日本金线鱼、红棘金线鱼亲缘关系较远。本研究为证实红棘金线鱼和苏门答腊金线鱼是2个不同的种提供了分子生物学的证据。In order to identify the phylogenetic relationship among three Nemipterus species （N. japonicus, N. netaurus and iV. mesoprion） caught from Beibu Gulf, we amplified their mitochondrial cytochrome oxidase subunit I （COI） gene fragments by PCR, and examined 696 base-pair nucleotide sequences of COI. The results show that all 48 sequences are grouped into 29 haplotypes and there are 104 variable nucleotide positions in COI gene fragments. Haplotypes analysis indicates that iV. japonicus and N. nemurus have obvious gene flow and closer rela- tionship and have no significant genetic difference based on sequence divergences and genetic distances analysis. Molecular phylogenetic trees are constructed with Scolopsis taenioptera and Eucinostomus currani as outgroups using maximum-parsimony and neighbor-joining, which suggests that the three Nemipterus species cluster into two clades. Specifically, N. japonicus and N. nemurus cluster into one clade, while N. mesoprion into another. Thus, it could be concluded that the relationship between N. japonicus and N. nemurus is so close that they may be the same species, while the relationship between N. mesoprion and N. japonicus and that between N. mesoprion and N. nemurus in pairwise is far, which provides molecular biology evidence that iV. nemurus and N. mesoprion are two different species.

关 键 词：金线鱼属 MTDNA COI 亲缘关系 序列分析 北部湾 

分 类 号：S917[农业科学—水产科学]