中国和匈牙利白斑狗鱼群体遗传变异的微卫星标记分析  

Genetic variability analysis of Chinese and Hungarian northern pike (Esox lucius L.) based on microsatellite markers

作　　者：王军[1] 吴惠仙[1] 杨新鑫[1] 马玉清[1] 钱龙[2] 李思发[1] 王成辉[1] 

机构地区：[1]上海海洋大学农业部水产动物种质资源与利用重点开放实验室,上海201306 [2]新疆生产建设兵团水产技术推广站,新疆乌鲁木齐830002

出　　处：《中国水产科学》2011年第3期531-536,共6页Journal of Fishery Sciences of China

基　　金：新疆生产建设兵团项目(2006GG18);上海市科委支援西部项目(09325800700)

摘　　要：本研究应用18个微卫星分子标记,对白斑狗鱼(Esox lucius L.)3个中国新疆群体(乌伦古湖、吉力湖和6号湖)和1个匈牙利巴拉顿湖群体的遗传结构进行了分析。结果表明,3个中国白斑狗鱼群体的平均等位基因丰富度(AR)、平均观测杂合度(HO)和平均期望杂合度(HE)均显著低于匈牙利巴拉顿湖群体(P<0.05),而匈牙利群体的平均近交系数(FIS)高于中国群体;经SMM和TPM模型检测,匈牙利白斑狗鱼群体存在显著的遗传瓶颈信号(P<0.001);AMOVA和群体两两比较的FST值表明,中国与匈牙利白斑狗鱼的遗传分化十分显著(P<0.01);NJ树、主成分分析(PCA)进一步证实中国白斑狗鱼与匈牙利白斑狗鱼群体间存在显著的遗传差异和分化。此外,贝叶斯遗传聚类结果表明,中国新疆6号湖白斑狗鱼群体极可能来源于乌伦古湖,而非吉力湖。Genetic variability of northern pike （Esox lucius L.） was investigated in three populations （Ulungur Lake, Jili Lake and No.6 Lake） in Xingjiang, China and one population （Balaton Lake） in Hungary by using 18 microsatellite markers. The results indicated that the mean allele richness （AR）, mean observed heterozygosity （Ho） and expected heterozygosity （HE） of the Chinese populations were significantly lower than those of the Hungarian population （P〈0.05）, whereas the mean inbreeding coefficient （FIs）of the Hungarian population was higher than that of the Chinese populations. Significant bottleneck signal was detected from Hungarian population by using SMM and TPM model （P〈0.001）. Analysis of molecular variance and pairwise FsT values indicated that there was distinct genetic difference between Chinese and Hungarian populations （P〈0.01）. The results of Neighbour-joining tree, Principal Coordinate Analysis （PCA） further confirmed that there was obvious genetic difference between Chinese and Hungarian populations. Furthermore, the results of Bayesian cluster analysis showed that northern pikes in the No. 6 Lake would have been introduced from the Ulungur Lake rather than from the Jili Lake.

关 键 词：白斑狗鱼 微卫星 遗传结构 瓶颈效应 

分 类 号：S917[农业科学—水产科学] Q959.46[生物学—动物学]