pH胁迫对中国明对虾抗氧化系统酶活力及基因表达的影响  

Effects of pH stress on antioxidant system enzyme activities and gene expression of Fenneropenaeus chinensis

作　　者：王芸[1,2] 李健[2] 李吉涛[2] 何玉英[2] 常志强[2] 刘德月[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071

出　　处：《中国水产科学》2011年第3期556-564,共9页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目(40706052);农业部公益性行业专项(200803012;201103034);国家虾产业技术体系项目(CARS-47);国家863计划项目(2010AA10A401);科技部农业科技成果转化资金项目(2010GB23260589)

摘　　要：研究了pH胁迫对中国明对虾Fenneropenaeus chinens＆）抗氧化系统的影响，将中国明对虾暴露于pH7．0、9．0的水体中148h，于胁迫后0、3、12、24、48、72、96、120、148h测定鳃、肝胰腺、肌肉和血淋巴总抗氧化活力（T-AOC）、抗超氧阴离子活力、过氧化氢酶（CAT）活力和CAT、过氧化物还原酶（Prx）基因表达的影响，以pH8．2作为对照。结果显示，pH胁迫12～24h，对虾各组织T-AOC、抗超氧阴离子、CAT酶活力及CAT基因表达均增加，pH胁迫120~148h上述指标受到抑制。对虾肝胰腺和肌肉P麒基因表达随胁迫时间增加逐渐升高，鳃和血淋巴Prx基因表达随胁迫时间增加呈下降趋势。高pH（9．0）胁迫时，对虾鳃抗氧化系统酶活力及基因表达水平达峰时问较其他3个组织明显缩短；而低pH（7．0）胁迫时，对虾肝胰腺抗氧化系统酶活力比其他组织变化快。实验结果表明，pH胁迫3～24h对中国明对虾抗氧化系统酶活力及相关基因表达有一定的诱导效果，但胁迫120～148h则抑制其抗氧化系统功能，可能会导致中国明对虾机体的氧化损伤；中国明对虾鳃和肝胰腺分别对高、低pH胁迫较敏感。Chinese shrimp, Fenneropenaeus chinensis, is an important mariculture species in China. Recently deteriorated pond environment due to intensive culture caused increased incidence of stress-induced diseases. It is well known that environmental stress can induce oxidative stress. However, there is little information about the effects of pH changes on antioxidant system of Chinese shrimp. In this study, Chinese shrimps were exposed to water of pH7.0 and 9.0 for 148 h and pH8.2 was set as control. Total antioxidation activity（T-AOC）, anti-superoxide anion activity, catalase （CAT） activity and the expression CAT, peroxiredoxin （Prx） gene were analyzed in gill, hepatopancreas, muscle and haemocytes of Chinese shrimp after 0 h, 3 h, 12 h, 24 h, 48 h, 72 h, 96 h, 120 h and 148 h exposure. The T-AOC, anti-superoxide anion activity, CAT activity and the gene expression of CAT in different tissues increased within 12-14 h of pH stress. However, they were inhibited in the extending periods of pH stress time. The Prx gene expression in heaptopancreas and muscle gradually increased, but decreased in gill and haemocytes with pH stress time prolonging. The enzyme activities and gene expression of an- tioxidant system in gills first reached its highest level in pH 9.0 group, faster than other tissues in 9.0 group. And the antioxidant enzyme activities in hepatopancreas in pH 7.0 group changed faster than in other tissues. The results indicate that the antioxidant enzyme activities and gene expression of Chinese shrimp were induced by short-term pH stress which is the response＂ to oxidative stress, while they were inhibited with pH stress time pro- longing. It was shown that long time neutral and alkaline-induced oxidative stress probably caused the antioxidant defense system injury in Chinese shrimp. Gills and hepatopancreas could be the sensitive tissues to high and low pH stress, respectively.

关 键 词：中国明对虾 PH胁迫 总抗氧化能力 抗超氧阴离子能力 过氧化氢酶 过氧化物还原酶 

分 类 号：S917[农业科学—水产科学]