吉富罗非鱼IGF-1基因的基因型对生长和体型的影响  

Effects of IGF-1 genotype on weight gain and body shape in GIFT strain Oreochromis niloticus

作　　者：阮瑞霞[1] 俞菊华[2] 李红霞[2] 李建林[2] 唐永凯[2] 

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,江苏无锡214081

出　　处：《中国水产科学》2011年第3期682-688,共7页Journal of Fishery Sciences of China

基　　金：农业部公益性行业科研专项(200903046-02);农业部淡水生物多样性保护与利用重点开放实验室第七期开放课题(LFBU0706);中央级公益性科研院所基本科研业务费专项资金项目(2011JBFC03)

摘　　要：通过比对6尾吉富罗非鱼(Genetically Improved Farmed Tilapia,GIFT)的胰岛素样生长因子1(Insulin-like Growth Factor 1,IGF-1)基因外显子1-部分内含子2和外显子3-部分内含子4序列,共找到3个SNPs位点,分别为内含子1_A1307G、内含子1_G1319T和内含子3_C6T。使用PCR-RFLP法检测了5个家系共121尾吉富罗非鱼在内含子1_A1307G和内含子3_C6T的基因型,分析不同基因型与生长性状的相关性。结果表明,内含子1_A1307G与雌鱼增重和体厚/体长值均显著相关(P<0.05),但与雄鱼的相关性不显著(P>0.05);内含子3_C6T与雄鱼和雌鱼的增重分别呈显著(P<0.05)和极显著相关(P<0.01),与雌鱼体高/体长显著相关(P<0.05)。为了验证所选标记在其他家系的表现,本实验检测了来自60个家系的417尾吉富罗非鱼在内含子1_A1307G、内含子3_C6T的基因型与增重性状的相关性,结果具有相同趋势。本实验筛选到与吉富罗非鱼体型和增重相关的SNP位点2个,可作为辅助育种标记。Insulin-like Growth Factor 1 （IGF-1） is one of important genes controlling growth and development in animals. By comparing with exonlpartial intron 2- exon 3- partial intron 4 sequences of IGF-1 from six individuals of Genetically Improved Farmed Tilapia（GIFT） strain, we identified three SNPs on IGF-1 in this study, including intron I_A 1307G, intron I_G 1319T and intron3_C6T. PCR-RFLP was performed in intron I_A 1307G and intron3_C6T genotypes detection on 121 individuals from five families of GIFT strain. The results of correlation analysis showed that intronl_A1307G was significantly associated with female fish weight gain and the ratio of body thickness/length （P〈0.05）. The weight gain and thickness/length ratio of female fish with AA genotype were significantly higher than those of female fish with GG type （P〈0.05）. However, the correlation of genotypes with those traits in male fish was not significant（P〉0.05）. Intron3_C6T was significantly associated with weight gain in both male （P〈0.05） and female （P〈0.01）. The weight gain of male fish with TT and CT genotype was significantly higher than that of male fish with CC type, while the weight gain of female with CT genotypes was significantly higher than that of female fish with TT. Intron3 C6T was also significantly associated with height/length ra- tio（P〈0.05） in female fish. The height/length ratio of female fish with TT and CT genotypes was significantly higher than that with CC（P〈0.05）. In order to verify the performance of the screened markers in other families, we detected the correlation between intronl_ A1307G, intron3_C6T genotypes and weight gain among 417 individuals from 60 families of GIFT strain（averaging seven individuals per family）, and similar results were observed. The diverse genetic background and smaller size of samples resulted in the decreased correlation significance. These results showed that genetic background and sample size should be considered during molecular maker screening.

关 键 词：胰岛素生长因子-1 吉富罗非鱼 单核苷酸多态性 增重 体型 相关性 

分 类 号：S917[农业科学—水产科学] Q959.483[生物学—动物学]