不同流式细胞分析系统对外周血淋巴细胞亚群分析结果的影响  

Effects of different analytic systems on the immunophenotypic analysis of peripheral blood lymphocyte subsets by flow cytometry

作　　者：黄春梅[1] 郭野[1] 陈倩[1] 刘定华[1] 崔巍[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,100730

出　　处：《中华检验医学杂志》2011年第5期403-408,共6页Chinese Journal of Laboratory Medicine

基　　金：卫生部临床检验标准化专业委员会基金资助项目

摘　　要：目的探讨部分临床实验室FCM淋巴细胞亚群分析参考范围应用的合理性及不同生产厂家的流式细胞仪和试剂组合对淋巴细胞亚群分析结果的影响。方法根据国内临床实验室常用的3个流式细胞仪型号（BeckmanCoulterEpicsXL、Beckman Coulter CytomicsFC500、BDFACSCalibur），分别选取3家北京地区临床流式细胞室（A、B、C室），按照各室的实际检测方案分别测定50份健康人静脉血标本，以验证各室淋巴细胞亚群分析参考范围是否合理。调查3家实验室室内全血质控品使用情况，并将商品化全血质控品分发各室，按照各自的实际实验方案在20个工作日内与常规标本平行处理、检测和分析。针对不同生产厂家的试剂，在同一流式细胞仪（型号为BeckmanCoulterEpicsXL）上用a、b、e、d4种不同的试剂组合对20份患者标本进行检测，其中试剂组合a为美国BeckmanCoulter公司同厂配套试剂和仪器，试剂组合b、c、d的检测结果分别与试剂组合a比较，计算b、C、d试剂组合偏倚〉10％的概率。采用相同试剂和溶血素（美国BeckmanCouher公司）对24份患者标本进行前处理，分别在2台不同厂家和型号的流式细胞仪（型号为BeckmanCoulterEpicsXL和BDFACSCalibur）上检测，比较相同试剂处理标本后不同仪器对淋巴细胞亚群分析结果的影响。采用同厂配套试剂和仪器，比较BeckmanCoulterEpicsXL和BDFACSCalibur两个流式细胞检测系统对20份患者标本检测结果的影响。结果A室的自然杀伤（NK）细胞及cD；T淋巴细胞／CD8T淋巴细胞（Td／T8），B、C两室的T4均有大于10％的结果落在相应的参考范围之外，超出相应参考范围的概率分别为16％（9／50）、24％（12／50）、22％（11／50）、12％（6／50）。3家实验室20个工作日内的室内质控均在参考范围内。与试剂组合a比较，试剂组合b、C的所有项目偏倚均较大，其中偏倚〉10％的概�Objective To investigate the appropriate setting up of normal reference ranges of lymphocyte subsets in some flow cytometry laboratories and to study the effects of different flow cytometers and various reagents by different manufacturers on the analysis of peripheral blood lymphocyte subsets. Methods Three FCM labs （named A, B and C ） in Beijing region were selected representing 3 commonly used flow cytometers （ Beckman Coulter Epics XL, Beckman Coulter Cytomics FC500, BD FACS Calibur）. 50 samples from healthy donors were distributed to 3 labs and tested according to individual lab＇s standard operating procedure to verify whether the normal reference ranges of peripheral blood lymphocyte subsets established were appropriate. The application of internal quality control was also investigated. Commercial blood quality control reagents were given to the 3 FCM labs and tested within 20 working days paralleled with routine samples. In addition, 20 patients＇ samples were prepared using 4 different combinations of reagents （ a , b , c and d）. The results from combination a, which used the Beckman Coulter reagents and instrument, were compared to the results from combination b, c and d, which used reagents from different manufacturers. Then the prepared samples were tested on Beckman Coulter Epics XL to evaluate the effects of different combinations of reagents on the results of peripheral blood lymphocyte subsets analyzed by the same instrument. Furthermore, 24 patients＇ samples prepared by same reagents from Beckman Coulter company were tested on both Beckman Coulter Epics XL and BD FACS Calibur respectively to assess the effects of different instruments on peripheral blood lymphocyte subsets. 20 patients＇ samples prepared by same reagents and instruments were analyzed by Beckman Coulter Epics XL analytic system and BD FACS Calibur analytic system respectively to assess the effects of the two analytic systems on the lymphocyte subsets. Results Over 10% of the results for NK and T4/T8 in lab A as wel

关 键 词：流式细胞术 淋巴细胞亚群 参考值 质量控制 

分 类 号：R446.11[医药卫生—诊断学]