芩丹胶囊对高血压血管外膜重构及TGF-β1/Smad信号转导通路的影响  

Effect of Qindan capsule on vascular adventitial remodeling and the TGF-β1/Smad signaling pathway

作　　者：葛汝青[1] 张继东[1] 黄山英[2] 王博[1] 任敏[1] 冯利[1] 郭雪峰[1] 吕怡静[1] 

机构地区：[1]山东大学齐鲁医院中医科,济南250012 [2]山东大学齐鲁医院教育部和卫生部心血管重构和功能研究重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2011年第4期7-12,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助项目(30873324);山东省科技发展计划(2010GWZ20242)

摘　　要：目的观察芩丹胶囊(QD)的降压作用以及对血管外膜TGF-β1/Smad信号转导通路的影响,探讨QD改善高血压血管外膜重构的作用机制。方法将老年自发性高血压大鼠(SHR)随机分为模型组(SHR组)、QD大剂量组(SHR+QDH组)、QD小剂量组(SHR+QDL组)、氯沙坦组(SHR+Los组),另设老年Wistar-Kyoto(WKY)大鼠空白对照组(WKY组)及正常用药组(WKY+QDH组)。给药组分别灌胃给予相应药物,SHR组和WKY组灌胃给予等量生理盐水。测量各组大鼠收缩压;免疫组化法观察TGF-β1和Smad7在胸主动脉外膜的蛋白表达;苦味酸-天狼猩红染色,偏振光显微镜下观察Ⅰ、Ⅲ胶原在胸主动脉外膜的表达;Image Pro Plus图像分析系统进行定量分析。结果与SHR组相比,SHR+QDH组、SHR+QDL组和SHR+Los组收缩压均下降(P<0.05);TGF-β1表达降低,Smad7表达增高(P均<0.05);外膜Ⅰ、Ⅲ胶原蛋白表达下降(P<0.05)。SHR+QDH组比SHR+QDL组效果更好(P<0.05)。结论 QD不仅能够有效降低SHR血压,而且能够干扰TGF-β1/Smad信号转导通路,从而抑制动脉血管外膜Ⅰ、Ⅲ胶原的表达,改善和逆转血管外膜重构。Objective To observe the effects of Qindan Capsule（QD） on the TGF-β1/Smad Signaling Pathway and its effect on blood pressure to investigate its mechanism to improve the remodeling of vascular adventitia.Methods Spontaneously hypertensive rats（SHR） were divided into the model group（SHR group）,the QD high dosage group（SHR＋QDH group）,the QD low dosage group（SHR＋QDL group）,and the losartan group（SHR＋Los group）.At the same time,16 Wistar-Kyoto（WKY） rats of the same age were the normal control（WKY group） and the QD group（WKY ＋QDH group）.All rats were administered with corresponding medicine or normal saline and their blood pressures were measured.The level of TGF-β1 and Smad7 in the thoracic aorta were detected by immune-histochemical staining.Ⅰ,III collagen were stained with picric-sirius red and observed under a polarizing microscope.The data were quantified by Image-Pro Plus.Results Compared with the SHR group,the blood pressure of the SHR＋QDH group,the SHR＋QDL group and the SHR＋Los group were reduced（P0.05）,with decreased expression of TGF-β1（P0.05）, increased Smad7（P0.05）,and decreased expression of Ⅰ,Ⅲ collagen（P0.05）.The SHR＋QDL group was better than the SHR＋QDL group（P0.05）.Conclusion QD can not only reduce blood pressure in SHR,but also can interfere with the TGF-β1/Smad Signaling Pathway by inhibiting the expression of Ⅰ,Ⅲ collagen in the arterial adventitia.Therefore,it can improve and even reverse the remodeling of vascular adventitia.

关 键 词：芩丹胶囊 血管外膜重构 TGF-Β1/SMAD Ⅰ、Ⅲ胶原 

分 类 号：R544.1[医药卫生—心血管疾病] R285.5[医药卫生—内科学]