氯胺酮对食蟹猴大脑血管病理及NADPH氧化酶表达的影响  

Effects of ketamine on cerebrovascular pathology and NADPH oxidase activation in cynomolgus monkeys

作　　者：朱薇[1,2] 潘芳[1] 江虹[1] 于洪鸾[1] 孙琳[1] 王力[1] 

机构地区：[1]山东大学医学院心理学研究所,济南250012 [2]山东医学高等专科学校,济南250002

出　　处：《山东大学学报（医学版）》2011年第4期47-51,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨氯胺酮连续给药对食蟹猴大脑动脉(动脉环交通支)形态及烟酰胺腺嘌呤二核苷酸磷酸盐(NADPH)氧化酶表达的影响。方法随机将12只雄性食蟹猴分为生理盐水组(4只)和氯胺酮组(8只,1 mg/kg),6个月后处死动物,采用HE染色法观察大脑动脉的形态变化,比色法测定H2O2的水平,Western blot法和免疫组织化学染色检测血管中NADPH氧化酶的表达水平。结果连续注射氯胺酮6个月后,NADPH氧化酶表达增加,血清和血管组织内活性氧(ROS)水平明显升高,大脑动脉形成血栓及炎症反应。结论氯胺酮诱导食蟹猴脑血管疾病的发生与NADPH氧化酶表达增加具有相关性。Objective To explore the effect of long-term administration of ketamine on cerebrovascular（communicating branches of the cerebral arterial circle） pathology and nicotinamide adenine dinucleotide phosphate（NADPH） oxidase activation in cynomolgus monkeys.Methods Twelve male monkeys were randomly divided into the experimental group and the control group,six in each group.Monkeys in the experimental group were injected with ketamine at 1 mg/ kg,while the control group received normal saline.6 months later,the monkeys were sacrificed.Cerebrovascular pathology was observed by HE staining.Reactive oxygen species（ROS） level was expressed as the decrement of H2O2 with the biochemical method.NADPH oxidase activation was observed by immunohistochemistry and Western blot.Results The experimental group showed a significant increase in NADPH oxidase activation and ROS level compared with the control group.Thrombus and inflammatory cells infiltration were observed in the cerebrovascular tissue of the experimental group.Conclusion Long-term administration of ketamine up-regulate NADPH oxidase level leading to cerebrovascular pathological changes.

关 键 词：氯胺酮 成束猴 NADPH氧化酶 活性氧 

分 类 号：R395.1[哲学宗教—心理学]