A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue  

作　　者：ZHANG Zhen LIU JingFu YAO Yan JIANG GuiBin 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100086, China [2]Department of Isotope, China Institute of Atomic Energy, Beijing 102413, China

出　　处：《Chinese Science Bulletin》2011年第15期1543-1547,共5页

基　　金：supported by the National Natural Science Foundation of China (21025729 and 20921063)

摘　　要：A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+) and samarium (Sm 3+),respectively.The detection limits for CAP and RAC were 0.06 and 0.25ng/g.The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5ng/g,and 69.8%-85.8% for RAC at spiking levels of 1-10ng/g.The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS,with correlation coefficients (R) between 0.92-0.98.A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol （CAP） and ractopamine （RAC） residues in 18 swine tissue samples, using anti-CAP and anti-RAC monoclonal antibodies labeled with europium （Eu^3＋） and samarium （Sm3＋）, respectively. The detection limits for CAP and RAC were 0.06 and 0.25 ng/g. The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5 ng/g, and 69.8%-85.8% for RAC at spiking levels of 1-10 ng/g. The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS, with correlation coefficients （R） between 0.92-0.98.

关 键 词：时间分辨荧光 莱克多巴胺 同时测定 猪组织 氯霉素 双标签 竞争力 免疫分析方法 

分 类 号：S859.84[农业科学—临床兽医学]