盐单胞菌属一新种的分离与鉴定  

Isolation and Identification of a New Species of Halomonas

作　　者：朱凤玲[1,2] 曲凌云[1,2] 洪旭光[1,2] 赖其良[3] 张进兴[1,2] 孙修勤[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]国家海洋局海洋生态环境科学与工程重点实验室,山东青岛266061 [3]国家海洋局第三海洋研究所海洋生物遗传重点实验室,福建厦门361005

出　　处：《海洋科学进展》2011年第2期221-228,共8页Advances in Marine Science

基　　金：国家科技基础平台工作项目--近海海洋微生物资源的标准化整理整合与共享(2004DKA30640);国家自然科学基金项目--海洋多重耐药菌新种DQHS4分类地位的确定及其耐药基因结构和水平传递的分析(41006103);国家海洋局第一海洋研究所基本科研业务费专项资金项目--海洋多重耐药菌新种DQHS4分类地位确定及其耐药基因结构和水平传递的分析(GY02-2011G24)

摘　　要：对即墨市大桥盐场的底泥样本进行微生物的可培养研究,分离出1株革兰氏阴性的中度嗜盐菌YC-SA28T。16S rRNA序列比对及系统发育分析结果表明,YCSA28T可能为γ-变形菌纲盐单胞菌属的新种,通过系统分类学研究发现,YCSA28T的16S rRNA序列与亲缘关系最近的盐单胞菌属模式菌株Halomonas ventosaeAl12T同源性为96.9%,其GenBank注册号为FJ984862。YCSA28T的荷兰细菌保藏中心受理序号为NCCB100305T,中国普通微生物保藏管理中心受理序号为CGMCC 1.9150T。YCSA28菌落为乳黄色,细胞杆状或短杆状,周生鞭毛。生长温度范围为5~40℃,生长pH值范围为6.0~9.0,生长绝对盐度（NaCl）范围为2%~15%。氧化酶/过氧化氢酶阳性。辅酶Q-9为主要的呼吸醌,主要脂肪酸为C18：1ω7c（42.88%）,C16：0（23.05%）及C16：1ω7c/ω6c（18.00%）。菌株YCSA28T染色体DNA的x（G＋C）为63.7%~63.9%,与模式菌株Halomonas vento-saeAl12T间的DNA-DNA杂交率为（45±3）%。结合各种形态及生理生化特征可知,YCSA28T应为盐单胞菌属一新种,建议命名为大桥盐单胞菌（Halomonas daqiaonensissp.nov.）。A moderately halophilic Gram-negative bacteria YCSA28 is isolated from a sedimentary sample at Daqiao saltern in Jimo to study the microorganism cultivation.It is learnt from the 16S rRNA sequence comparison and the systematic growth analysis that YCSA28 can be a noval species of the Halomonas in the Gammaproteobacteria class.As it is shown in its taxological study,YCSA28 has the similarity about 96.9% to its nearest neighour of Halomonas ventosae A112T.A GenBank accession number,FJ984862,is given to the strain.It has been already received and numbered not only as NCCB100305T by NCCB but also as CGMCC 1.9150T by CGMCC.Its colonies appear in cream-yellow.It is in a rod or short rod shape,and with lateral flagella.Growth temperature is between 5~40℃,pH value within 6~9,and growth salinity（NaCl） within 2%~15 %.It is oxidase/catalase-positive.The major quinone of Strain YCSA28 is Q-9,and the cellular fatty acids mainly consist of C18：1ω7c（42.88%）,C16：0（23.05%） and C16：1ω7c/ω6c（18.00%）.The x（G＋C） content of the chromosomal DNA is 63.7%-63.9%.DNA-DNA hybridization between the YCSA28 strain and H.ventosae Al12T has 45±3 % relatedness.It is learnt from the morphological features combined with the physiological and biochemical features that Strain YCSA28 represents a novel species of the genus Halomonas.The name Halomonas daqiaonensis sp.nov.is proposed for Strain YCSA28T.

关 键 词：盐单胞菌 新种 鉴定 

分 类 号：Q93-331[生物学—微生物学]