补肾固齿丸对大鼠牙周组织表达基质金属蛋白酶13的影响  

Effects of Bushenguchiwan on expression of matrix metailoproteinase-13 in rats＇ periodontium

作　　者：文钦[1] 杨恒[1] 丁一[1] 

机构地区：[1]四川大学华西口腔医学院牙周科,成都610041

出　　处：《中华口腔医学杂志》2011年第5期280-285,共6页Chinese Journal of Stomatology

摘　　要：目的研究补肾固齿丸对大鼠牙周组织表达骨胶原酶基质金属蛋白酶13（matrixmetalloproteinase-13，MMP-13）的影响，探讨补肾固齿丸治疗牙周炎的机制。方法将成功建立牙周炎模型的64只大鼠按照随机数字表法分为4组：牙周炎模型组、补肾固齿丸低剂量组（0．8g·kg-1·d-1）、中剂量组（2g·kg－1·d-1）和高剂量组（4g·kg-1·d-1），各组大鼠均为16只。16只正常大鼠作为正常对照组。采用免疫组化染色法，以积分吸光度值作为参数进行半定量测定MMP－13在大鼠牙周组织的表达是否具有差异。结果建模成功后牙周炎建模大鼠牙周组织的MMP-13呈阳性表达，而正常大鼠牙周组织中未见表达。相对于牙周炎模型组（积分吸光度值为5．3233±0．7993），经过高、中、低剂量补肾固齿丸治疗30d后，牙周组织中MMP-13积分吸光度值均下降（分别为2．9103±0．5534、3．6588±0．4330、4．4550±0．4255，P〈0．05），给药60d后，补肾固齿丸高、中、低剂量组MMP-13的积分吸光度值（分别为2．1855±0．5381、2．8558±0．4759、3．8980±0．5885）较牙周炎模型组（6．2693±0．4538）下降更明显（P〈0．05）。结论补肾固齿丸可以下调骨胶原酶MMP-13的表达，且补肾固齿丸高剂量组（4g·kg-1·d-1）的效果更佳。Objective To investigate the influence of Bushenguchiwan on expression of matrix metalloproteinase-13 （MMP-13） in periodontium of rats with experimental periodontitis. Methods The model of experimental periodontitis of rats was established and treated by Bushenguehiwan with different doses. The periodontal tissues from groups of different doses were immunohistochemically stained by antibody of MMP-13. The expression of MMP-13 was examined and semi-quantitative analysis of signals performed by integrated absorbance. Results MMP-13 was intensely positive in gingival epithelial cells and periodontal fibroblasts in periodontitis models and negative in normal rat periodontal tissues. After 30 days of Bushenguchiwan treatment with high dose, middle dose and low dose, the expression of MMP-13 （2. 9103 ± 0. 5534,3. 6588 ± 0. 4330, 4. 4550 ± 0. 4255 ） was down-regnlated respectively compared with model rats （5. 3233 ±0. 7993） ,P 〈 0. 05. After 60 days of treatment the expression of MMP-13 （2. 1855 ±0. 5381, 2. 8558 ± 0. 4759,3. 8980± 0. 5885 ） was down-regulated more significantly, with model rats （ 6. 2693 ± 0. 4538） ,P 〈 0. 05. Conclusions Bushenguchiwan could down-regulate the expression of MMP-13 in rats＇ periodontium and the high dose group had better effect.

关 键 词：大鼠 基质金属蛋白酶13 补肾固齿丸 实验性牙周炎 

分 类 号：R276.8[医药卫生—中医五官科学]