二烯丙基硫醚对微囊藻毒素LR致细胞毒性及DNA损伤的影响  

Effects of diallyl sulfide on cytotoxicity and oxidative DNA damage induced by microcystin-LR

作　　者：农清清[1] Toru Takeuchi Kimiko Izumo 张志勇[1] 何敏[3] 

机构地区：[1]广西医科大学公共卫生学院职业卫生与环境卫生学教研室,广西南宁530021 [2]日本鹿儿岛大学环境医学研究室 [3]广西医科大学公共卫生学院职业-卫生与环境卫生学教研室,广西南宁530021

出　　处：《毒理学杂志》2011年第2期84-86,共3页Journal of Toxicology

基　　金：中国博士后科学基金(特别资助200902355;面上资助20080440816);广西教育厅科研立项项目(200911LX38)

摘　　要：目的研究大蒜主要活性成分之一的二烯丙基硫醚(diallyl sulfide,DAS)对微囊藻毒素LR(MCLR)诱导的细胞毒性和氧化性DNA损伤的影响。方法体外培养人肝癌细胞株HT17,用1μmol/L MCLR染毒的同时给予DAS(0.02～2 mmol/L)作用24 h,同时设立未处理对照组、单纯MCLR染毒组和单纯DAS处理组。测定乳酸脱氢酶(LDH)漏出率来反映细胞毒性,并应用免疫酶染色法检测8-羟基脱氧鸟嘌呤核苷(8-OHdG)水平。结果单纯MCLR染毒组的LDH漏出率和8-OHdG染色强度明显高于未处理对照组。0.2 mmol/L以上的DAS与MCLR联合处理细胞时,LDH漏出率和8-OHdG染色强度明显低于单纯MCLR染毒组。结论 DAS可能对MCLR诱导的细胞毒性和氧化性DNA损伤具有保护作用。Objective To study the effects of diallyl sulfide（DAS） on cytotoxicity and oxidative DNA damage induced by microcystin-LR（MCLR）.Methods Human hepatoma cell lines HT17 were treated with medium alone or 1 μmol/L MCLR in the presence or absence of DAS（0.02 ～ 2 mmol/L） for 24 h,then the cytotoxicity was measured by LDH release assay,and oxidative DNA damages were detected using immunostaining of 8-hydroxydeoxiguanosine（8-OHdG）.Results LDH release and the levels of 8-OHdG in MCLR-treated cells were higher than that of untreated cells.DAS at concentrations higher than 0.2 mmol/L significantly inhibited the LDH release and 8-OHdG formation induced by MCLR.Conclusion The results suggested that DAS may protect against the cytotoxicity and oxidative DNA damage induced by microcystin-LR.

关 键 词：二烯丙基硫醚(DAS) 微囊藻毒素LR 细胞毒性 氧化性DNA损伤 

分 类 号：R994[医药卫生—毒理学]