苯并(a)芘对人支气管上皮细胞端粒长度的影响  

Effects of benzo(a)pyrene on telomere length in human bronchial epithelial cells

作　　者：宾萍[1] 段化伟[1] 王亚东[1] 戴宇飞[1] 牛勇[1] 刘清君[1] 陈泓[1] 刘庆[1] 郑玉新[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《毒理学杂志》2011年第2期87-90,共4页Journal of Toxicology

基　　金：国家自然科学基金(30901178;30625031);中国疾病预防控制中心职业卫生与中毒控制所青年科技基金(No.09G0503)

摘　　要：目的体外探讨苯并(a)芘[Benzo(a)pyrene,B(a)P]对人支气管上皮细胞端粒长度的影响。方法应用人支气管上皮细胞株16HBE,给予不同浓度的B(a)P处理,采用定量PCR方法测定细胞全基因组DNA的相对端粒长度,观察细胞全基因组DNA相对端粒长度的变化情况。以4μg/ml博来霉素(Bleomycin,BLM)为阳性对照。结果与0μmol/L组比较,1和4μmol/L B(a)P染毒组,以及阳性对照组的相对端粒长度较短,差异有统计学意义(P<0.05);而16μmol/L B(a)P染毒组与0μmol/L组比较,差异无统计学意义(P>0.05)。结论 B(a)P可对人支气管上皮细胞16HBE的全基因组DNA端粒长度产生影响,在一定剂量内会导致细胞全基因组DNA端粒长度缩短。Objective To investigate the effects of Benzo（a）pyrene[B（a）P]on telomere length in human bronchial epithelial cells in vitro.Methods Human bronchial epithelial cell lines（16HBE） were treated with B（a） P in vitro.Real-time polymerase chain reaction（RT-PCR） was used to evaluate the telomere length of genomic DNA in cells,and the changes of relative telomere length were marked.The positive control was 4 μg/ml Bleomycin（BLM）.Results Compared to the group of 0 μmol/L B（a）P,the groups of 1 μmol/L,4 μmol/L and positive control group,the telomere length were significantly shorter（P 0.05）,but no significant difference was found between group of 16 μmol/L B（a）P and control.Conclusion The data showed that B（a）P had influence on the telomere length of genomic DNA in 16HBE cells.It might lead to short telomere length in a certain range of dosage.

关 键 词：苯并(A)芘 端粒长度 定量PCR 

分 类 号：R135[医药卫生—劳动卫生]