机械性创伤致继发性肺损伤大鼠肺组织中叉头盒转录因子A1表达的变化及意义  

Effect of secondary lung injury caused by mechanical trauma on the expression change of forkhead box protein A1 at both mRNA and protein levels

作　　者：戴文娟[1] 刘卓拉[1] 贾国平[1] 高晓玲[1] 

机构地区：[1]山西医科大学第二医院呼吸内科,太原030001

出　　处：《中国医药》2011年第6期662-664,共3页China Medicine

基　　金：山西医科大学第二医院博士基金（20090407）

摘　　要：目的 研究机械性创伤致继发性肺损伤大鼠肺组织中叉头盒转录因子（Fox）A1表达的变化及意义。方法将雄性Wistar大鼠40只完全随机分为伪创伤组和创伤组，每组20只。利用Noble-Collip创伤仪制备机械性创伤模型。全部大鼠均于造模后6h腹主动脉采血处死，提取肺组织的总RNA和总蛋白。逆转录聚合酶链反应检测FoxAlmRNA的表达，蛋白质印迹检测FoxAl蛋白的表达。结果创伤组FoxAlmRNA表达水平高于伪创伤组（P〈0．01），是伪创伤组的9．25倍；FoxAl蛋白表达：伪创伤组（0．59±0．04），创伤组（0．91±0．03），创伤组FoxAl蛋白表达水平高于伪创伤组，差异有统计学意义（P〈0．01）。结论FoxAl在机械性创伤致继发性肺损伤中起着重要的作用。Objective To study the effect of secondary lung injury caused by mechanical trauma on the expression change of Forkhead box protein A1 （ FoxA1 ） at both mRNA and protein levels. Methods Forty male Wistar rats were randomly divided into sham group and trauma group, each group 20, Noble-Collip drum was used to establish mechanical trauma rat model. The total RNA and protein of lung tissue was extracted after modeling of ab- dominal aortic blood sampling time points were killed. The expression of FoxA1 mRNA in rats＇ lung tissue was observed by real-time RT-PCR, the expression of FoxA1 protein was detected by Western Blot. Results The expression of FoxA1 protein in trauma group was higher than that in sham group（ sham group 0.59 ±0.04, trauma group 0.91 ± 0.03, P 〈 0.01 ）. Conclusion FoxA1 plays an important role in secondary lung injury caused by meehanical trauma.

关 键 词：机械性创伤 急性肺损伤 叉头盒转录因子A1 

分 类 号：R641[医药卫生—外科学]