碱灰化法和氯酸消化法测定人乳汁含碘量的对比研究  

A comparative study on alkaline ashing method and chloric acid digestion method for determination of human milk iodine

作　　者：孙毅娜[1] 董金茹[2] 范同梅 李永梅[1] 叶艳[1] 林来祥[1] 阎玉芹[1] 陈祖培[1] 刘守军[3] 

机构地区：[1]天津医科大学内分泌研究所卫生部激素与发育重点实验室,300070 [2]天津市河西区妇产科医院 [3]哈尔滨医科大学中国疾病预防控制中心地方病控制中心

出　　处：《中国地方病学杂志》2011年第3期342-344,共3页Chinese Jouranl of Endemiology

基　　金：基金项目：国家科技支撑计划课题（2006BA106805）

摘　　要：目的 以碱灰化法作金标准,探讨氯酸消化法测定人乳汁含碘量的准确性.方法 收集天津市河西区新鲜人乳汁样品61份,分别应用2008年发布的〈食物中碘的测定砷铈催化分光光度法〉中的碱灰化法和1999年发布的〈尿碘的砷铈催化分光光度测定方法〉中的氯酸消化法测定其含碘量.结果 采用两种检测方法得到61对人乳汁含碘量数据,两种方法的测定结果呈高度正相关（r=0.960,t=26.3,P〈O.01）,其回归方程为（Y）=-28.1＋0.808X（X为自变量,即碱灰化法测定结果;（Y）为因变量,即氯酸消化法测定的估计值）;两种方法测定结果的平均差值为68.3μg/L,氯酸消化法要比碱灰化法平均低38.9%,经配对t检验,其差异具有统计学意K（t=11.3,P〈0.01）.乳汁样品稀释3、4、5倍后,经氯酸消化澄清率分别为80.3%（49/61）、95.1%（58/61）、100.0%（61/61）.碱灰化法和氯酸消化法测定61份人乳汁含碘量,中位数分别为165.4、110.0μg/L.结论 与碱灰化法比较,氯酸消化法测定结果明显偏低,提示该方法存在系统误差,不能代替碱灰化法.Objective Take alkaline ashing method as golden standard to explore the accuracy of chloric acid digestion method in determination of human milk iodine. Methods Sixty one breast milk samples collected in Hexi district of Tianjin was measured by the method for determination of iodine in foodstuff by As3＋-Ce4＋ catalytic spectrophotometry （referred to as the alkaline ashing method） published in 2008 and the method for determination of iodine in urine by As3＋-Ce4＋ catalytic spectrophotometry（referred to as acid digestion） published in 1999, respectively. were highly correlated（r = 0.960, t = 26.3, P 〈 0.01）, and the regression equation was （Y） = - 28.1 ＋ 0.808X, in which X was independent variable, that is the results of alkaline ashing method; （Y） was dependent variable, that is the estimated data of chloric acid digestion method. The average difference of the results measured by the two methods was 68.3 μg/L, and the results from chloric acid digestion was 38.9% which lower than that of alkaline samples were diluted by 3,4 and 5-fold and then digested by chloric acid, the liquid clarification rates were 80.3% ashing and chloric acid digestion method were, respectively, 165.4, 110.0 μg/L. Conclusions Compared with alkaline ashing method, the results determined by chloric acid digestion method are significantly lower. It is suggested that there are systemic errors in chloric acid digestion method, which means that alkaline ashing method can not be replaced by the chloric acid digestion method.

关 键 词：碘 乳 人 分光光度法 

分 类 号：R446[医药卫生—诊断学] R15[医药卫生—临床医学] O[理学]