微弧氧化处理的内植物在骨质疏松兔中的生物学表现  

Biological performance of micro-arc oxidative implants in the osteoporotic rabbits

作　　者：石磊[1] 王陵[2] 张扬[1] 刘帅[1] 吕荣[1] 王军[1] 雷伟[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]第四军医大学统计教研室,西安710032

出　　处：《中国矫形外科杂志》2011年第10期836-839,共4页Orthopedic Journal of China

基　　金：国家高技术研究发展计划(863计划)(编号:2007AA02Z468)

摘　　要：[目的]观察经过微弧氧化(micro-arc oxidation,MAO)表面处理的内植物是否能提高其在骨质疏松兔体内生物学表现。[方法]选用30只成年雌性新西兰大白兔,随机分为卵巢切除组(OVX组,n=20)和假手术组(Sham组,n=10),手术后2周,OVX组接受甲泼尼龙注射,Sham组接受生理盐水注射,6周后分别行双能X线检测两组的骨密度(bone mineral density,BMD)。确定OVX组骨质疏松模型建立成功后,兔胫骨两侧随机置入经MAO处理的及未处理的内植物,处理组为实验组,未处理组为对照组,术后12周处死动物,取材标本进行生物力学实验,显微CT检测以及组织学分析。[结果]OVX组的BMD显著低于Sham组(P<0.05)。内植物置入OVX组12周后,实验组的最大拔出力,能量吸收值,骨体积和组织骨密度,新骨形成率和骨接触率均优于对照组(P<0.05)。[结论]微弧氧化处理能够提高内植物在骨质疏松条件下的生物学表现,有利于增强内植物的稳定性。[Objective]To observe whether the micro-arc oxidative（MAO） implant can improve its biological performance in osteoporotic rabbits.[Method]Twenty-four adult female rabbits were divided randomly in to two group：ovariectomized group（OVX group,n=20）,and unovariectomized group（Sham group,n=10）.Two weeks after operation,the OVX group was operated with methylprednisolone and the sham group injected with physiological saline.At 6 weeks,the bone mineral density（BMD） was detected by dual energy X-ray absorptiometry in both groups.After osteoporosis was confirmed in OVX group,two kinds of implants were randomly placed in each tibia of the rabbits（experimental group：MAO-implant,control group：untreated implant）.All the animals were sacrificed at 12 weeks after implantation.Biomechanical testing,micro-CT analysis,and histological observation were administered.The data were statistically analyzed.[Result]The BMD was significantly decreased in the OVX group compared to the sham group（P0.01）.At 12 weeks after implantation in OVX group,the max pull-out strength,absorbed energy,bone volume,tissue mineral density,area ratio of newly formed bone area to the whole area and length ratio of direct bone-implant interface to total implant surface in the experimental group were significantly enhanced compared with the control group（P0.01）.[Conclusion]MAO treatment improved the biological performance and stability of implants in osteoporosis.

关 键 词：微弧氧化 骨质疏松 内植物 内植物-骨界面 骨形成 

分 类 号：R687.3[医药卫生—骨科学]