PLGA-TCP-BMP-2人工骨结合带血供自体骨修复骨缺损  

An experiment research of the composite of BMP-2 and PLGA/tricalcium phosphate with vascularized ulna in repairing the large segmental defect of sheep radius

作　　者：周密[1] 徐建强[1] 李长庚[1] 张树明[1] 王长江[1] 王克利[1] 朱方正[1] 

机构地区：[1]第二炮兵总医院骨科,北京100088

出　　处：《中国矫形外科杂志》2011年第10期846-848,共3页Orthopedic Journal of China

基　　金：全军十五医药卫生科研基金课题(编号:06G018)

摘　　要：[目的]研究PLGA-TCP-BMP-2人工骨结合自体带血供自体尺骨移植修复大段骨缺损的效果。[方法]手术造成30 mm绵羊桡骨骨缺损,A组置入PLGA-TCP-BMP-2人工骨及带血运的长段尺骨,B组仅置入PLGA-TCP-BMP-2人工骨,C组不置入任何材料。3组均以钢板固定骨缺损区。术后24周拍摄X线片并处死动物,进行组织学观察评价。[结果]X线检查示A组桡骨缺损处完全成骨修复,皮质骨与髓腔的轮廓清晰;B组亦能完全修复,但新生骨密度及髓腔轮廓清晰度均不如A组;C组无有效骨痂形成。组织学检查示A、B组骨痂外层形成为板层骨,C组缺损区可见大量纤维组织填充。[结论]PLGA-TCP-BMP-2人工骨结合自体带血供长段尺骨移植能够很好的修复绵羊桡骨30 mm的骨缺损。[Objective]To study the effects of the composite of PLGA-TCP-BMP-2 artificial bone with vascularized ulna in repairing the large segmental defect of sheep radius.[Method]A 30 mm defect was made in middle segment of sheep radius.In group A,the composite of BMP-2 and PLGA/tricalcium phosphate with vascularized ulna was implanted into the radial defect.PLGA-TCP-BMP-2 artificial bone was implanted into the defect in group B.No material was implanted in group C.Plates were used to fix the bone in both groups.X ray was used to evaluate the repairing effect at 24 weeks postoperatively.Animals were sacrificed at 24 weeks after operation to make histological analysis.[Result]X ray revealed that the bone defect of radius was fully repaired at 24 weeks after operation with clear contour of cortical bone and medullary cavity in group A.While in group B,the contour was not as clear as in group A.No effective callus was found in group C.Histological study showed that the callus had already formed compact bone which integrated with broken ends of fractured bone in group A and B.Large amount of bone marrow could be seen in the middle part and no residual material was found.However,large amount of fibrous tissue was found in bone defect area in group C.[Conclusion]The composite of BMP-2 and PLGA/tricalcium phosphate with vascularized ulna could repair large segmental defect of sheep radius satisfactorily.

关 键 词：自体骨 BMP-2 PLGA 磷酸三钙 骨缺损 

分 类 号：R687.3[医药卫生—骨科学]