抑制一氧化氮合成对孕鼠血管调节因子的影响  

The Effect of Inhibition of Nitric Oxide Synthesis on Vasoregulatory Factors in Pregnant Rats

作　　者：马成斌[1] 庄依亮[1] 许元初 杨幼明[3] 

机构地区：[1]上海医科大学妇产科医院,200011 [2]上海市吴淞中心医院妇产科 [3]上海医科大学,200011

出　　处：《中华妇产科杂志》1999年第9期521-524,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　研究抑制一氧化氮（ Ｎ Ｏ） 合成对孕鼠血浆内皮素（ Ｅ Ｔ） 、血栓烷素（ Ｔ Ｘ Ａ２） 、前列环素（ Ｐ Ｇ Ｉ２） 及血管紧张素 Ｉ Ｉ（ Ａ Ｎ Ｇ Ｉ Ｉ） 水平变化的影响；并探讨这些因子在妊高征发病中的作用。方法　选择清洁级 Ｗｉｓｔａｒ 大鼠，随机分为两组，（１） 对照组１２ 只，于妊娠第１４ 天开始皮下注射生理盐水直至分娩。（２） 亚硝基左旋精氨酸甲酯（ Ｌ Ｎ Ａ Ｍ Ｅ） 组，１２ 只，于妊娠第１４ 天开始皮下注射一氧化氮合酶（ Ｎ Ｏ Ｓ） 抑制剂 Ｌ Ｎ Ａ Ｍ Ｅ，每日１２５ ｍｇ／ｋｇ 体重，直至分娩。测定两组孕鼠尾动脉收缩压、尿蛋白、血小板数及胎鼠胎盘重量。测定两组血浆 Ｎ Ｏ、 Ｅ Ｔ、 Ｐ Ｇ Ｉ２ 、 Ｔ Ｘ Ａ２ 及 Ａ Ｎ Ｇ Ｉ Ｉ水平。结果　 Ｌ Ｎ Ａ Ｍ Ｅ 组孕鼠出现类妊高征特征，表现为血压明显升高至（１４０ ．６ ±３ ．８）ｍ ｍ Ｈｇ（１ｍ ｍ Ｈｇ ＝ ０ ．１３３ｋ Ｐａ） ，尿蛋白含量增加为（８０１ ．３８ ±５７ ．１２）ｍｇ／ Ｌ，血小板数显著减少至（５３３ ±４２） ×１０９／ Ｌ，及胎鼠胎盘重量明显减轻。对照组孕鼠血压（１０８ ．６ ±３ ．４）ｍ ｍ Ｈｇ ，尿蛋白（４１３ ．２２ ±８５ ．５５）ｍｇ／ Ｌ，血小板?Objective To study the effect of the inhibition of nitric oxide(NO)synthesis on plasma endothelin (ET),prostaglandlin I 2(PGI 2),thromboxane (TXA 2)and angiotensin (ANGII) in pregnant rats, and to investigate the role of these factors in the pathogenesis of pregnancy induced hypertension(PIH). Methods Pregnant rats were divided into two groups randomly.Saline solution or L nitro arginine methyl ester (L NAME) 125 mg/day was given subcutaneously from day 14 of gestation till delivery.Systolic blood pressure, urine protein, platelet count,and weight of pups and placentae were determined. Plasma NO, ET, PGI 2, TXA 2 and ANGII were measured by RIA. Results Pregnant rats which were given L NAME produced physical signs similar to those of PIH,such as increase in systolic blood pressure (140.6±3.8) mmHg and urine protein (801.38±57.12)mg/L, and decrease in platelet count (533±42)×10 9/L and weight of pups and placentae.Compared with controls, plasma NO, PGI 2 and ANG II were decreased significantly while plasma ET and TXA 2 were increased significantly in rats given L NAME. Conclusion Inhibition of NO synthesis can cause imbalance of several vaso regulatory factors, which may be responsible for the pathogenesis of PIH.

关 键 词：一氧化氮 妊娠高血压 综合征 孕鼠 

分 类 号：R714.246[医药卫生—妇产科学]