正常早孕蜕膜非免疫细胞免疫活性的实验研究  

Research on Immunological Activity of Non immunological Cells of Decidua in Normal Early Pregnancy

作　　者：胡冬梅[1] 陈竹钦[2] 颜建华[2] 曹咏清[1] 王妮[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室,100080 [2]第三军医大学大坪医院妇产科

出　　处：《中华妇产科杂志》1999年第9期533-535,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：中国科学院动物研究所计划生育生殖生物学国家重点实验室资助

摘　　要：目的　了解蜕膜中非免疫细胞的免疫活性。方法　取蜕膜中非免疫细胞培养的上清液，用放射免疫方法（ Ｒ Ｉ Ａ） 测定其对人外周血 Ｔ 细胞的转化、自然杀伤（ Ｎ Ｋ） 细胞的杀伤率、淋巴细胞分泌白细胞介素２（ Ｉ Ｌ２） 及 Ｂ 细胞分泌 Ｉｇ Ｇ 的影响。结果　蜕膜非免疫细胞培养的上清液可使 Ｔ 细胞、 Ｎ Ｋ 细胞、 Ｂ 细胞的免疫学活性不同程度地受到抑制，其最大抑制率分别为２２ ．７ ％ 、５２ ．３ ％ 和１４ ．８ ％ ，但对淋巴细胞 Ｉ Ｌ２ 的分泌无显著改变。结论　蜕膜非免疫细胞具有免疫抑制功能，这有助于防止母体对胎儿产生免疫排斥，保证妊娠的建立与进行。Objectives To investigate the immunological activity of non immunological cells of decidua in normal early pregnancy. Methods Harvest the supernatant from cultured non immunological cells of decidua. Inspect the effects of the supernatant on transforming function of T cells, killing activity of natural killer cells, the IgG secretion of B cells and the interleukin 2 (IL 2) secretion by lymphocytes of human peripheral blood by radioimmunological assay (RIA). Results The supernatant of the cultured non immunological cells of decidua can inhibit the immunological function of T cells, natural killer cells and B cells to different extents, their maximum inhibiting ratio were 22.7%, 52.3% and 14.8% respectively, but there is no significant effect on the IL 2 secretion by lymphocytes. Conclusions The non immunological cells of decidua in normal early pregnancy have immunological inhibiting function, which might contribute to prevent fetus from being expelled by maternal immunological system.

关 键 词：妊娠初期 蜕膜 免疫活性 淋巴细胞转化 

分 类 号：R714.14[医药卫生—妇产科学]