阻塞性睡眠呼吸暂停综合征患者内源性大麻素系统合成及降解酶水平  

A study on the endogenous cannabinoid system synthetic and catabolic enzyme levels in patients withobstructive sleep apnea

作　　者：武月青[1] 王蓓[1] 宋璐[1] 王强[1] 陈晓阳[1] 刘卓拉[1] 

机构地区：[1]山西医科大学第二医院呼吸科,太原030001

出　　处：《中华结核和呼吸杂志》2011年第5期359-361,共3页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：基金项目：山西省留学归国人员科研项目（201014100）

摘　　要：目的观察阻塞性睡眠呼吸暂停综合征（OSA）患者内源性大麻素系统（ECS）合成及降解酶的变化。方法对2009年7月至12月在山西医科大学第二医院睡眠中心就诊的患者行整夜多导睡眠监测（PSG），实时PCR法检测外周血单个核细胞上花生四烯酸乙醇胺（AEA）及2-花生四烯酸甘油（2-AG）的合成及降解酶水平，了解OSA病情严重程度对其的影响。结果与正常对照组相比，OSA组ECS中两种酶N-酰基磷脂酰乙醇胺水解磷脂酶D（NAPE）、单酰基甘油脂肪酶（MAGL）的表达有差异（P〈0．05）。MAGL与最长呼吸暂停时间（LA）、最低血氧饱和度（LSaO：）、微觉醒指数（MAI）存在相关（r值为0．34、-0．31和0．24，均P〈0．05）。与单纯OSA组相比，合并高血压的OSA患者FAAH表达有差异（P〈0．05）。结论OSA可影响ECS合成酶及降解酶的表达，使内源性大麻素物质升高。Objective To observe the differences of endogenous cannabinoid system （ECS） synthetic and catabolic enzyme levels between the obstructive sleep apnea syndrome （OSA） patients and the control subjects. Methods Patients with OSA confirmed by PSG in our Sleep Center were randomly recruited from July to December, 2009. Peripheral blood was obtained to isolate mononuclear cells and the mRNA levels of anandamide （AEA） synthase N-acylphosphatidylethanolamine hydrolyzing phospholipase D （NAPE） , fatty acid amide hydrolase （FAAH） and 2-arachidonoylg lycerol （2-AG） synthase diacylglycerol lipase（DAGL） and hydrolase monoacylglycerol lipase （MAGL） were measured by real-time polymerase chain reaction. The association between the severity of OSA and the enzyme levels were explored. Results There was a significant difference in both the NAPE and MAGL levels between patients with OSA and the control subjects. The level of MAGL was related to some indices of severity of OSA , including the longest apnea time , lowest blood oxygen saturation and the micro-arousal index （ r = 0. 31,0.24, 0. 34, respectively, all P 〈 0. 05 ）. Compared with patients with OSA alone, patients with OSA complicated by hypertension showed a different level of FAAH （ P 〈 0. 05 ）. Conclusion OSA altered the expression of the ECS synthetic and catabolic enzymes, leading to an increase in endogenous cannabinoid substances.

关 键 词：睡眠呼吸暂停综合征 内源性大麻素系统 

分 类 号：R766[医药卫生—耳鼻咽喉科]