Identification and Characterization of 43 Microsatellite Markers Derived from Expressed Sequence Tags of the Sea Cucumber (Apostichopus japonicus)  

作　　者：JIANG Qun LI Qi YU Hong KONG Lingfeng 

机构地区：[1]Fisheries College, Ocean University of China, Qingdao 266003, China

出　　处：《Journal of Ocean University of China》2011年第2期185-190,共6页中国海洋大学学报（英文版）

基　　金：supported by the grants from the Science and Technology Development Program of Shandong Province (2009GG10005013);Doctoral Program of Ministry of Education of China (20090132110018)

摘　　要：The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags （ESTs） downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs （8.18%） and 299 simple sequence repeats （SSRs） were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.

关 键 词：sea cucumber Apostichopus japonicus MICROSATELLITE EST 

分 类 号：Q78[生物学—分子生物学] S511[农业科学—作物学]