自体成肌细胞移植改善兔心肌梗死后心功能的实验研究(英文)  

Autologous myoblasts transplantation improves heart function after myocardiac infarction

作　　者：庄炜[1] 李莉[1] 林国强[1] 邓震宇[1] 

机构地区：[1]中南大学湘雅医院心胸外科,长沙410008

出　　处：《中南大学学报（医学版）》2011年第4期286-293,共8页Journal of Central South University ：Medical Science

基　　金：supported by the Department of Science and Technology of Hunan Province,P.R.China(2010SK3095)

摘　　要：目的:将体外培养并纯化的自体骨骼肌成肌细胞植入心肌梗死区,观察其心脏功能有无改善及改善程度,以探讨成肌细胞移植治疗的机制。方法:采用反复差速贴壁法体外培养、纯化及鉴定新西兰兔自体成肌细胞。结扎冠状动脉致局部心肌梗死后2周,分别将成肌细胞和对照的细胞培养液注入心肌梗死区,4周后观察心功能改善情况,鉴定心肌梗死边缘区毛细血管密度及植入细胞的形态。结果:体外培养获得高纯度(85.4%±6.7%)Desm in阳性的自体骨骼肌成肌细胞。经Buxco系统有创心功能测定显示自体成肌细胞移植组较对照组的左心室等容收缩期室内压最大上升速率[+dp/dtmax,(1 217.77±89.91)mmHg/s vs.(897.83±70.04)mmHg/s]和左心室等容舒张期室内压最大下降速率[-dp/dtmax,(-1 174.58±91.5)mmHg/s vs.(-753.67±69.66)mmHg/s]均有所改善。免疫组织化学和电镜证实植入的细胞为肌源性且细胞移植区域的毛细血管密度较对照组高。结论:采用反复差速贴壁法培养可在体外获得高纯度骨骼肌成肌细胞,植入成肌细胞能增加心肌收缩力,改善心功能并促进心肌梗死区域的毛细血管数量增加。Objective To determine whether autologous skeletal myoblasts implantation improves the cardiac function after myocardial infarction and the possible mechanism. Methods Myocardial infarction was induced by ligation of the left anterior descending coronary artery in rabbits. At 2 weeks, 1.34 x 10^7 to 1.75 × 10^7 autologous skeletal myoblasts were infused into the lesion via direct intramuscular injection. In the control group, the postinfarction hearts were infused with medium alone. Buxco invasive cardiac function testing and histopathological examination were utilized to evaluate the functional and structural changes in the myocardium 4 weeks later. Results Both maximum rising rate of the left intraventricular pressure [ ＋ dp/dtmax, （ 1 217.77 ± 89.91 ） mmHg/s vs. （897.83 ± 70.04） mmHg/s ] and maximum falling rate of the left intraventricular pressure [ - dp/dtmax, （ -1 174.58±91.5） mmHg/s vs. （ -753.67 ± 69.66）mmHg/s] were improved in the myoblast transplanted group compared with medium infusion group. The positive desmin immunostaining skeletal myofibers in the myocardium were found throughout the infraeted areas and the border zone. Conclusion Autologous skeletal myoblasts can establish muscle tissue when transplanted into oostinfarction hearts, and this mucle can treat mvocardiac infarction effectively.

关 键 词：细胞移植 成肌细胞:心肌梗死 

分 类 号：R542.22[医药卫生—心血管疾病]