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作 者:王娅丽 王锦秀 常红宇 倪细炉[1,3] 田英[1,3] 秦彬彬
机构地区:[1]宁夏林业研究所股份有限公司,银川750004 [2]西北特色经济林栽培与利用国家地方联合工程研究中心,银川750004 [3]种苗生物工程国家重点实验室,银川750004
出 处:《植物研究》2011年第3期330-335,共6页Bulletin of Botanical Research
基 金:宁夏自然科学基金项目(NZ09192);西北特色经济林栽培与利用国家地方联合工程研究中心资助项目(2010-EFC-2)
摘 要:为探讨枸杞新品系NQ-2纯系种植结实率低的原因,采用TTC法、联苯胺—过氧化氢法、花粉—胚珠比、杂交指数和套袋试验等方法,对NQ-2花粉活力、柱头可授性及繁育系统等进行了研究。结果表明:NQ-2单花花期为3~4 d,花后8 h散粉结束,开花当天花粉活力最高,花粉寿命可持续9 d以上;开花前一天柱头已具可授性,开花第1天柱头可授性最强,开花第3天柱头基本失去可授性;枸杞新品系NQ-2平均花粉-胚珠比8615,OC I=4,结合人工授粉实验结果确定其繁育系统为专性异交。自交不亲和是导致NQ-2纯系种植落花落果结实率低的主要原因。To explore the factors that cause the low fruit setting rate of pure line planting of Lycium barbarum NQ-2,the flowering characteristic,pollen viability,stigma receptivity and breeding system of L.barbarum NQ-2 were studied by means of observation on pollen viability,stigma receptivity,pollen-ovule ration,out-crossing index,emasculation,bagging and artificial pollination using Ningqi 1 as control.The results showed that:(1)The florescence of individual last 3-4 days,the pollen viability was high during the 1st-3rd day,then began to decline,the life span of pollen was more than 9 days;(2)The stigma has receptivity one day before flowering,the receptivity was strongest on the 1st day,and on the 3rd day stigma lost its receptivity;(3)The pollen-ovule ratio(p/o) is 8615,out crossing index is 4;Based on the result of emasculation,bagging and artificial pollination studies,the breeding system of NQ-2 should belongs to obligate xenogamy.The self-incompatibility is the main reason for the low fruit setting rate of pure line planting of NQ-2.
关 键 词:枸杞新品系NQ-2 花粉活力 柱头可授性 杂交指数 花粉—胚珠比 授粉实验 繁育系统
分 类 号:S567.2[农业科学—中草药栽培]
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