食管后迷走神经干切断对大鼠小肠ghrelin受体表达的影响  

Effect of retroesophageal truncal vagotomy on the expression of ghrelin receptor 1a in the small intestine in rats

作　　者：杨成广[1] 袁青领[1] 王志刚[1] 

机构地区：[1]上海交通大学附属第六人民医院普外科,上海200233

出　　处：《中国现代普通外科进展》2011年第4期260-264,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:研究食管后迷走神经干切断对大鼠小肠肌层ghrelin受体变化的影响。方法:采用小肠壁全层石蜡切片免疫组化受体染色、小肠肌层免疫荧光受体染色和免疫印迹技术检测迷走神经后干切断24h后小肠肌层ghrelin受体的表达。结果:石蜡切片受体染色,受体荧光染色和免疫印迹受体表达量检测均显示食管后迷走神经后干切断后小肠肌层ghrelin受体表达量呈下调趋势。结论:食管后迷走神经干切断对大鼠小肠ghrelin受体表达量有影响,使ghrelin受体表达量下调。Objective： To investigate the effect of retroesophageal truncal vagotomy on the expression of ghrelin receptor（GHS-R1a） in the small intestine in rats.Methods： The expressions of ghrelin receptors（GHS-R1a） in small intestinal muscle layers at 24h after retroesophageal truncal vagotomy were detected by ways of full-thick small intestinal wall paraffin sections immunohistochemistry receptors staining,small intestinal muscle layers immunofluorescent staining and Western blot.Results： The results of paraffin sections receptors staining,immunofluorescent staining and Western blot indicated that the expressional trend of ghrelin receptors was down-regulated after retroesophageal truncal vagotomy.Conclusion： Retroesophageal truncal vagotomy could affect the expression of ghrelin receptors,the down-regulation of ghrelin receptors in small intestinal muscle layers was observed.

关 键 词：GHRELIN 小肠动力 ghrelin受体 迷走神经切断术 

分 类 号：R656.7[医药卫生—外科学]