扁柑种质资源亲缘关系的ISSR分析  

ISSR Analysis of Citrus reticulata Blanco cv. Bian Gan Germplasm Resources

作　　者：郭丽英[1] 黄桂香[1] 何新华[1] 张树伟[1] 李峰[1] 陈虎[1] 

机构地区：[1]广西大学农学院,广西南宁530004

出　　处：《热带作物学报》2011年第3期443-446,共4页Chinese Journal of Tropical Crops

基　　金：科技部国际合作项目(No.2008DFA30900);广西研究生创新项目(No.2008105930901D2;1105930903050)

摘　　要：为了准确了解广西扁柑资源现状、类型、分类地位及亲缘关系,从而更好地开发利用,本试验对35份柑橘资源,其中包括21份广西扁柑资源、2份越南柑资源及12份近缘的其它柑橘属资源进行ISSR-PCR分析。结果显示:10条引物共扩增出134条DNA片段,其中108条具有多态性,比率为81%。用UPGMA软件进行聚类分析,35份柑橘资源的遗传系数在0.64～1.00之间,在遗传系数为0.80处将35份材料划分为5类,其中所有扁柑类资源均和椪柑、南丰蜜桔聚在一类,初步证明扁柑类资源的分类地位应属于宽皮柑橘类的桔类。各扁柑资源间的相似系数在0.90以上,亲缘关系较近。This study aimed at accurately understanding of resource status and types,taxonomic status and genetic relationship of Guangxi Citrus reticulata Blanco cv.Bian Gan,thus promoting its utilization.Thirty-five accessions of Citrus germplasm,including 21 Guangxi Citrus reticulata Blanco cv.Bian Gan species,2 Vietnam samples and 12 closely related varieties were analysed by using inter-simple sequence repeat（ISSR） and the results showed that 134 fragments of DNA were amplified with 10 primers by using ISSR-PCR,among which 108（81%） fragments were polymorphic.The genetic distance was analyzed,and a tree diagram constructed by using UPGMA algorithm.The genetic similarity coefficients among the accessions were calculated with NTSYS-pc2.10e software and the values were between 0.64 and 1.00.The 35 materials could be clustered into 5 groups at the similarity coefficient level of 0.80,all Citrus reticulata Blanco cv.Bian Gan samples had the greatest genetic similarity with Ponkan and Nanfeng mandarin,indicating that Citrus reticulata Blanco cv.Bian Gan was mandarin.Citrus reticulata Blanco cv.Bian Gan had a near relationship because of a high index which was above 0.90.

关 键 词：扁柑 种质资源 ISSR 聚类分析 

分 类 号：S666.9[农业科学—果树学]