番茄XRN2基因的克隆、表达分析及遗传转化  

Clonging,Expression Analysis and Genetic Transformation of XRN2 Gene in Tomato

作　　者：刘俊江[1] 李正国[1] 杨迎伍[1] 先志强[1] 邓伟[1] 

机构地区：[1]重庆大学生物工程学院基因工程研究中心重庆市高校功能基因与调控新技术重点实验室,重庆400030

出　　处：《热带作物学报》2011年第3期447-451,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.30972002;31071798);中央高校基本科研业务费(No.CDJXS102300)

摘　　要：为了研究番茄XRN2基因的功能,利用RT-PCR技术从番茄各组织混合cDNA中扩增了番茄XRN2基因全长编码序列,并对其进行了表达模式分析。结果表明该基因在番茄根部表达最强烈。通过对番茄叶片进行伤害诱导处理,发现番茄XRN2基因表达呈上升趋势。将该基因在组成型启动子CaMV35S驱动下定向构建至植物表达载体pCambia1301-35S,重组载体命名为pCambia1301-35S-XRN2和pCambia1301-35S-AsXRN2。通过农杆菌介导法转化获得转基因番茄,为进一步阐明XRN2基因在番茄中的功能奠定了基础。To understand the function of XRN2 gene in tomato,the full length SlXRN2 cDNA was amplified by RT-PCR from tomato mix cDNAs.Expression analysis results showed that SlXRN2 expressed the highest in tomato roots.The expression of XRN2 gene could be up-regulated by wound stress on the leaves.Then SlXRN2 gene driven by the constitutive promoter CaMV35S was directed cloning into plant expression vector pCambia1301-35S,named as pCambia1301-35S-XRN2 and pCambia1301-35S-AsXRN2.Transgenic tomato lines were obtained via agrobacterium-mediated transformation,which would provide significant evidence for further study of XRN2 function in tomato

关 键 词：XRN2 半定量PCR 伤害处理 载体构建 遗传转化 番茄 

分 类 号：S641.2[农业科学—蔬菜学] Q344.13[农业科学—园艺学]