单硝酸异山梨酯类制剂对急性心肌梗死血管内皮生长因子表达的作用  

The effect of isosorbide mononitratenitrate S. R. capsules on the expression of vascular endothelial growth factor in acute infarcted myocardium

作　　者：王静[1,2,3] 周玉杰[1,2] 葛海龙[1,2] 方哲[1,2] 韩红亚[1,2] 高霏[1,2] 王建龙[1,2] 

机构地区：[1]首都医科大学附属北京安贞医院 [2]北京市心肺血管疾病研究所干部病房,北京100029 [3]北京丰台医院急诊科

出　　处：《心肺血管病杂志》2011年第3期246-248,共3页Journal of Cardiovascular and Pulmonary Diseases

摘　　要：目的:结扎小型猪冠状动脉左前降支(left anterior descending,LAD)建立急性心肌梗死(a-cute myocardial infarction,AMI)模型,评价和探讨单硝酸异山梨酯类制剂对AMI后血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法:中华小型猪48只,全麻下开胸结扎LAD远段,建立急性心肌梗死(AMI)模型,术后随机分为实验组(24只)和对照组(24只),实验组口服长效硝酸酯类制剂(异乐定缓释胶囊,50 mg/d),对照组口服安慰剂,分别于服药后2 w、4 w、6 w和8 w取梗死区周围心肌组织标本,采用Western Blotting法及Realtime PCR法分别检测不同时间2组梗死区周围心肌组织VEGF表达水平。结果:与对照组相比,在4 w、6 w,实验组梗死区周围心肌组织VEGF表达水平均显著高于对照组[(1 819.0±408.4)vs.(1 247.5±356.8);(2 054.4±380.3)vs.(1 937.5±506.2),P<0.05]。结论:单硝酸异山梨酯类制剂能改善小型猪急性心肌梗死后,梗死区周围心肌组织VEGF表达水平,且以4 w、6 w时最为显著。Objective：To study the effect of isosorbide mononitratenitrate S.R.capsules on the expression of vascular endothelial growth factor in acute infarcted myocardium.Methods：Acute myocardial infarction was es-tablished through clipping left anterior descending（LAD） vessel of swine.After coronary artery was clipped,48 swines were randomized into 2 groups,one is therapeutic group,which fed 50mg isosorbide mononitratenitrate S.R.capsule daily,the other is control group.Every 6 swines in each group was evaluated at the 2nd week,the 4th week,the 6th week and the 8th week after surgery respectively.The endpoints of this study include the expression of vascular endothelial growth factor（VEGF） which evaluated by Western Blotting and Realtime PCR.Results：In therapeutic group,The VEGF expressed more in the therapeutic group on the 4th and 6th week [（1 819.0 ± 408.4） vs.（1 247.5 ±356.8）;（2 054.4 ±380.3） vs.（1 937.5 ±506.2）,P〈0.05].Conclusion：The VEGF expression is more in the therapeutic group than control groups on the 4th and 6th week.

关 键 词：心肌梗死 血管内皮生长因子 动物实验 猪 

分 类 号：R54[医药卫生—心血管疾病]