微波消化—固相萃取光度法测定中草药及制剂中的镍  

Method for determination of nickel(Ⅱ) contents in several Chinese herbal medicines by microwave digestion-solid phase extraction spectrephotometry

作　　者：吴方评[1] 蒲艳春[1] 杨勇[1] 姚祖福[1] 杨宏健[1] 

机构地区：[1]湖南省怀化医学高等专科学校,湖南怀化418000

出　　处：《微量元素与健康研究》2011年第3期46-48,共3页Studies of Trace Elements and Health

摘　　要：目的：选用2-（2′-喹啉偶氮）-5-二乙氨基苯胺（QADEAA）作为显色试剂,并研究了其与镍的显色反应。方法：在pH=6.0的六次甲基四胺—盐酸缓冲溶液介质中,SDS存在下,2-（2′-喹啉偶氮）-5-二乙氨基苯胺（QADEAA）与镍反应生成2∶1稳定配合物,该配合物可被Waters Porapak Sep-Pak-C18固相萃取小柱萃取,用异丙醇洗脱后用光度法测定。结果：异丙醇相中配合物λmax=595 nm,ε=1.38×105 L/mol/cm。镍含量在0~1.0μg/ml内符合比尔定律。结论：以上方法可用于中草药及制剂中镍元素含量的测定。Objective：Choosen 2-（2′-quinolinylazo）-5-diethlminoaniline（QADEAA） as a chromogenic reagent,the color reaction of 2-（2′-quinolinylazo）-5-diethlmino-aniline with nickel（Ⅱ） was studied.Method：In the medium of SDS and the presence of pH=6.0（NH2CH2）2CH-CH2（CH2NH2）-HCl buffer solution.QADEAA can reacts with nickel（Ⅱ） to form a stable 2∶1 complex.The colored complex can be extracted by C18 cartridge and eluted by isopropy lalcohol,then can be determined by spectrophotometry.Result：The molar absorptivity of the complex is 1.38×105L/moL/cm at 595 nm.Beer＇s law is obeyed in range of 0～1.0 μg/ml.Conclusion：This method can be applied to the determination of nickel（Ⅱ） in Chinese herbs.

关 键 词：2-(2′-喹啉偶氮)-5-二乙氨基苯胺 镍 固相萃取光度法 

分 类 号：R284.1[医药卫生—中药学]