毛细管电泳法测定黄芪注射液中的酚酸  

Simultaneous determination of phenioc acids in astragalus injection by capillary zone electrophoresis

作　　者：刘菲[1] 韩旭[2] 张伟东[1] 张红影[1] 李占华[1] 

机构地区：[1]河北医科大学,石家庄050091 [2]河北省疾病预防控制中心,石家庄050021

出　　处：《中国卫生检验杂志》2011年第5期1106-1107,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立了毛细管电泳-紫外检测法同时测定黄芪注射液中香草酸、阿魏酸、绿原酸含量的方法。方法:以30 mmol/L硼砂缓冲溶液(pH9.24)为电泳介质,检测波长为285 nm,分离电压为15 kV,进样时间20 s,峰面积定量。结果:香草酸、阿魏酸和绿原酸分别在0.4μg/ml^130.0μg/ml(R=0.9997)、0.8μg/ml^130.0μg/ml(R=0.9991)和1.0μg/ml^240.0μg/ml(R=0.9989)范围内呈良好的线性关系,检出限分别为0.12μg/ml、0.28μg/ml和0.31μg/ml,加标回收率均在95%以上。结论:该方法样品预处理简单,无需预富集,分离效果好,可用于实际样品的分析。Objective：To establish a method for determine vanillic,ferulic acid and chlorogenic acid in Astragalus injection by capillary zone electrophoresis.Methods： Under optimum conditions,the analytes could be separated and detected in a 30 mM tetraborate buffer（pH 9.24） within 10 min using a separation voltage of 15 kV at the detected wavelength of 285 nm.Results： A good linear relationship existed between peak area and concentration in the range of 0.4 μg/ml^130.0 μg/ml,0.8 μg/ml^130.0 μg/ml and 1.0 μg/ml^240.0 μg/ml for vanillic,ferulic and chlorogenic acid respectively.The detection limits,based on three times noise were 0.12 μg/ml,0.28 μg/ml and0.31 μg/ml.The average recovery was above 95%.Conclusion： The method has been used for the determination of phenioc acids in Astragalus injection,and the assay results were satisfactory.

关 键 词：黄芪注射液 酚酸 毛细管电泳 

分 类 号：O657.8[理学—分析化学]