低温磷酸处理-气相色谱法检测韭菜中有机磷农药  

Determination of organophosphorus pesticide residues in leek by Gas chromatography after cryogenic and phosphoric acid treatment

作　　者：洪萍[1] 徐陆妹[1] 李峰[1] 应杏秋[1] 韩见龙[2] 

机构地区：[1]杭州市萧山区疾病预防控制中心,浙江萧山311201 [2]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2011年第5期1112-1114,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立韭菜中10种有机磷农药残留检测新方法。方法:样品在低温冷冻状态下,加磷酸去除硫化物,乙腈提取,提取液挥干后丙酮溶解,过Na2CO3/CARB/NH2柱净化,DB-1701毛细管柱分离,FPD检测。结果:10种有机磷农药浓度在0.05μg/m l^1.00μg/m l范围内标准曲线线性相关系数在0.9996~1.0000之间,样品加标回收率为64.9%~106.3%,RSD为0.80%~5.8%。结论:本方法简便,净化效果好,可用于韭菜等含硫蔬菜有机磷农药残留的检测。Objective：To develop a new method to detect ten organophosphorus pesticides residue in leek.Methods： The frozen leek were treated with a phosphoric acid—assisted to inactivate enzymes,the samples were extracted with acetonitrile.The extract was cleaned up with Na2CO3/CARB/NH2 column and detected using gas chromatography with flame photometric detector（GC—FPD）.Results： The linear range was 0.05 μg/ml-1.00 μg/ml（r=0.9996-1.0000）.The recovery range was 64.9%-106.3%.RSD was 0.8%-5.8%.Conclusion： The method is simple and convenient for analyzing organophosphorus pesticide residues in Leek.

关 键 词：低温磷酸处理 韭菜 有机磷农药 气相色谱法 

分 类 号：O657.71[理学—分析化学]