缺氧条件下绒毛外滋养细胞中Cyr61和NOV mRNA表达研究  

The expression of Cyr61 and NOV mRNA in extravillous trophoblasts under hypoxia

作　　者：陈茜[1] 刘燕燕[1] 徐晓燕[1] 陈汉平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科优生与产前诊断实验室,武汉430030

出　　处：《中国优生与遗传杂志》2011年第6期33-34,62,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的检测CoC l2化学缺氧条件下,绒毛外滋养细胞中促血管生成蛋白Cyr61和NOV的转录水平表达情况,探讨其在子痫前期发病机制的研究。方法采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)技术检测绒毛外滋养细胞中Cyr61和NOV mRNA的表达。结果 (1)促血管生成蛋白Cyr61和NOV在绒毛外滋养细胞中表达。(2)与正常对照组相比,缺氧条件下Cyr61和NOV转录水平降低(P<0.05)。结论 Cyr61和NOV的表达下调可能与胎盘血管生成障碍有关,从而参与了子痫前期的发病有关。Objective：To detected expression levels of the proangiogenic protein Cyr61 and NOV in extravillous trophoblasts under hypoxia,using cobalt chloride（CoCl2） as hypoxic mimetic.Methods： Quantitative polymerase chain reaction（qRT-PCR） was implied to investigate expression of Cyr61 and NOV in extravillous trophoblasts.Results：（1） The proangiogenic protein Cyr61 and NOV were founded in extravillous trophoblasts.（2）Compared with control,expression levels of Cyr61 and Nov mRNA in trophoblast cells cultured under hypoxic condition（P0.05）.Conclusion： Down-regulation of Cyr61 and NOV might result in failure of placental vascular formation and play role in the pathogenesis of preeclampsia.

关 键 词：绒毛外滋养细胞 缺氧 富含半胱氨酸蛋白61 肾胚细胞瘤过表达 子痫前期 血管形成 

分 类 号：R714.244[医药卫生—妇产科学]