结核/非结核分枝杆菌核酸快速检测方法临床应用价值  

Clinical value of real-time FQ-PCR assay for detection of Mycobacterium tuberculosis/non-tuberculous mycobacteria

作　　者：康丽军[1] 洪峰[2] 冯建兵 刘威[1] 吴华[1] 易俊莉[2] 王甦民[2] 

机构地区：[1]北京老年医院,北京100095 [2]北京结核病控制研究所,北京100035 [3]北京洛奇临床检验所,北京102206

出　　处：《中国防痨杂志》2011年第5期263-266,共4页Chinese Journal of Antituberculosis

摘　　要：目的评价结核/非结核分枝杆菌核酸快速检测方法的临床应用价值。方法应用实时荧光PCR技术对420例标本进行临床试验研究,并与抗酸杆菌涂片和分枝杆菌培养方法对照比较。结果结核分枝杆菌核酸检测灵敏度为48.5%(其中菌阳标本灵敏度为95.8%,菌阴标本灵敏度为14.5%),特异度99.3%,阳性预测值为99.1%,阴性预测值为55.5%;临床试验388例痰标本中检测出1例非结核分枝杆菌核酸阳性,经测序确认,准确率100%;同时对32例非结核分枝杆菌临床分离株进行核酸检测,并经测序确认,准确率100%。结论应用实时荧光PCR技术,能实现在1支管内同时进行结核分枝杆菌/非结核分枝杆菌的核酸检测。该方法具有简单快速、特异性好污染性少的特点。可作为实验室辅助检测结核/非结核分枝杆菌核酸方法之一。Objective To evaluate the clinical diagnostic value of real-time FQ-PCR assay for the detection of Mycobacterium tuberculosis（MTB）/non-tuberculous mycobacteria（NTM）. Methods 420 specimens were detected by real-time FQ-PCR,and the results were compared with those of AFB smear and culture. Results The sensitivity of FQ-PCR was 48.5%,in which the sensitivity in bacterium-positive and bacterium-negative specimens were 95.8% and 14.5%,respectively.The specificity,positive predictive value and negative predictive value of FQ-PCR were 99.3%,99.1% and 55.5%,respectively.1 of 388 sputum specimens was NTM-positive by FQ-PCR,which was confirmed by DNA sequencing.32 NTM isolates were confirmed by FQ-PCR and DNA sequencing.Their accuracy was 100%. Conclusions The real-time FQ-PCR was a simple,rapid and specific assay for the detection of Mycobacterium tuberculosis/non-tuberculous mycobacteria in a sealed tube.

关 键 词：分枝杆菌 结核 分枝杆菌属 核酸类 聚合酶链反应 

分 类 号：R52[医药卫生—内科学]