两种结核分枝杆菌相关γ-干扰素定量检测试剂盒检测结核分枝杆菌感染结果比较研究  

A comparative study on two interferon-gamma quantitative detection kits for the diagnosis of tuberculosis

作　　者：李晓非[1] 赵勤[1] 汪亚玲[1] 余文林[1] 

机构地区：[1]云南省昆明市第三人民医院,昆明650041

出　　处：《中国防痨杂志》2011年第5期267-270,共4页Chinese Journal of Antituberculosis

基　　金：传染病重大专项(2008ZX10301)

摘　　要：目的评价新的国产结核分枝杆菌相关γ-干扰素定量检测试剂盒(TB-IGRA)——体外释放酶联免疫法诊断结核病的敏感性和特异性。方法采用TB-IGRA试剂盒对319例肺结核、23例肺外结核、39例排除结核的肺部疾病和104例健康人群的血清标本进行检测,同时与澳大利亚Cellestis公司的QuantiFERON-TB GOLDin tube(QFT-GIT)试剂进行平行比较分析。结果采用TB-IGRA试剂,检测肺结核病人的敏感性为90.9%,肺外结核的敏感性为78.3%,特异性为76.9%;采用QFT-GIT试剂,检测肺结核病人的敏感性为88.4%,肺外结核的敏感性为78.3%,特异性为80.4%,2种试剂进行平行比较,敏感性和特异性方面差异无统计学意义。结论 TB-IGRA试剂盒对诊断结核病有较高的敏感性和特异性,可用于结核病尤其是涂阴肺结核和肺外结核的辅助诊断。Objective To evaluate the sensitivity and specificity of interferon-gamma（IFN-γ） quantitative detection kit（TB-IGRA,Beijing Wantai Biological Pharmacy Corporation Ltd.） for the diagnosis of tuberculosis（TB）. Method We detected the serum specimens from 319 patients with pulmonary TB,23 patients with extrapulmonary TB,39 patients with non-TB lung disease and 104 healthy people with TB-IGRA and QuantiFERON-TB GOLD in tube（QFT-GIT,Cellestis,Australia） simultaneously,and compared their results. Results The sensitivities of TB-IGRA for TB and extrapulmonary TB were 90.9% and 78.3%,respectively,and it＇s specificity was 76.9%.The sensitivities of QFT-GIT for TB and extrapulmonary TB were 88.4% and 78.3%,respectively,and it＇s specificity was 80.4%.There was no significant difference between these two kits. Conclusion TB-IGRA is a highly sensitive and specific kit in the diagnosis of TB,and it can be used in the accessory diagnosis of tuberculosis,especially extrapulmonary tuberculosis.

关 键 词：结核/诊断 分枝杆菌 结核 干扰素Ⅱ型 试剂盒 诊断 敏感性与特异性 

分 类 号：R52[医药卫生—内科学]