产AmpC酶多重耐药不动杆菌的耐药性研究  

Analysis of resistant spectrums in acinetobacter producing AmpC β-lactamases

作　　者：张伟珍[1] 

机构地区：[1]浙江中医药大学附属第一医院检验科,杭州310006

出　　处：《浙江医学》2011年第5期646-648,677,共4页Zhejiang Medical Journal

摘　　要：目的 了解AmpC酶在多重耐药不动杆菌中的分布,分析其对β-内酰胺类抗生素的耐药情况.方法 采用K-B扩散法、三维试验、PCR法检测126株不动杆菌的耐药情况以及所产的AmpC酶.结果 126株不动杆菌中,经三维试验和PCR扩增AmpC基因确证产AmpC酶的有38株.产AmpC酶不动杆菌对亚胺培南的敏感率最高达40.1%,,对头孢吡肟的敏感率为30.9%,对羧苄西林、替卡西林、氨曲南、头孢他啶、头孢噻肟的耐药率均在90%左右,对氨苄西林均不敏感.结论 临床分离的不动杆菌以鲍曼不动杆菌为主,产AmpC酶的不动杆菌对三代头孢类抗生素、亚胺培南的耐药率较高.Objective To analysis the resistance to β-lactams in Acinetobacter and investigate the AmpC β -lactamases produced by Acinetobacter. Methods The K-B diffusion test,the three-dimensional extract test and PCR amplification were used to detect the AmpC β-lactamases produced by 126 Acinetobacter. Results 38 strains produced AmpC β-lactamases by the three-dimensional extract test and ampC gene amplification. The drug sensitivity rate to Imipenem and cefepime was 40.1% and 30.9%, the incidence of Ampicillin-resistant was100%. Conclusion The major β-lactamases were AmpC β-lactamases. The resistant rate of the third cephalosporin was 〉90%.

关 键 词：不动杆菌 Β-内酰胺酶 耐药机制 

分 类 号：R378[医药卫生—病原生物学]