基于竹膜固定酪氨酸酶的L-酪氨酸光度测定法  

Photometric Determination of L-Tyrosine Based on the Catalytic Action of Tyrosinase Immobilized on Inner Membrane of Bamboo

作　　者：李永进[1] 

机构地区：[1]长江大学生命科学学院,荆州434025

出　　处：《理化检验（化学分册）》2011年第5期530-532,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：长江大学博士启动基金资助

摘　　要：提出了将酪氨酸酶固定在戊二醛活化的竹膜上制成检测L-酪氨酸的单元,用于L-酪氨酸的光度测定。此方法系基于在pH 7.0的磷酸盐缓冲溶液中酪氨酸酶催化酪氨酸转化为多巴醌,多巴醌进一步与3-甲基-2-苯并噻唑啉酮腙盐酸盐（MBTH）反应生成栗色加合物。此化合物在490 nm波长处有特征吸收峰,并在此波长所测得的吸光度与L-酪氨酸的浓度在5~200μmol·L-1之间呈线性关系,其检出限（3S/N）为2.5μmol·L-1。应用此方法测定了复方氨基酸注射液中L-酪氨酸的含量,所得结果与该药品的标示值一致。It was proposed that tyrosinase was immobilized on the inner membrane taken from bamboo and activated by pentanedial to prepare a detection unit for photometric determination of L-tyrosine.The determination was based on the catalytic action of tyrosinase on the transformation of L-tyrosine to dopa-quinone which was in turn reacted with 3-methyl-2-benzothiazolinone hydrazone（MBTH） to give a chestnut colored additive product having its absorption maximum at 490 nm in a PBS of pH 7.0.Values of absorbance measured at this wavelength were found to keep linear relationship with concentration of L-tyrosine in the range from 5 to 200 μmol·L-1,with its detection limit（3S/N） of 2.5 μmol·L-1.The proposed method was applied to the determination of L-tyrosine in injections of compound amino acids the results obtained were in consistency with the labelled value.

关 键 词：光度法 竹膜 酪氨酸酶 L-酪氨酸 

分 类 号：O657.32[理学—分析化学]